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PMID: 9096308 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 7 ·1997-04-01 ·Pages 2843-7

Passner JM, Steitz TA

Abstract

The 2.2 A resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with cAMP and a 46-bp DNA fragment reveals a second cAMP molecule bound to each protein monomer. The second cAMP is in the syn conformation and is located on the DNA binding domain interacting with the helix-turn-helix, a beta-hairpin from the regulatory domain and the DNA (via water molecules). The presence of this second cAMP site resolves the apparent discrepancy between the NMR and x-ray data on the conformation of cAMP, and explains the cAMP concentration-dependent behaviors of the protein. In addition, this site's close proximity to mutations affecting transcriptional activation and its water-mediated interactions with a DNA recognition residue (E181) and DNA raise the possibility that this site has biological relevance.

MeSH Terms
Cyclic AMP/metabolism Cyclic AMP Receptor Protein/metabolism DNA/metabolism DNA-Binding Proteins/metabolism Molecular Sequence Data Molecular Structure Protein Binding
Chemicals
Cyclic AMP Receptor Protein DNA-Binding Proteins DNA Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Passner J M
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA.
Steitz T A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.7.2843
Published
1997-04-01
Pages
2843-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20284
Subset
IM
Grants
NIGMS NIH HHS · P01 GM022778 · United States
NIGMS NIH HHS · GM22778 · United States
Databases
PDB
2CGP
External Links
PubMed source DOI: 10.1073/pnas.94.7.2843 PMC Full Text View journal details
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