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PMID: 9094620 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human T-cell leukemia virus type 1 transactivator protein Tax colocalizes in unique nuclear structures with NF-kappaB proteins.

Journal of virology ·Vol. 71 ·No. 5 ·1997-05-00 ·Pages 3484-97

Bex F, McDowall A, Burny A, Gaynor R

Abstract

The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) is a potent activator of viral transcription. Tax also activates the expression of specific cellular genes involved in the control of T-lymphocyte growth via effects on cellular transcription factors, including members of the NF-kappaB/cRel family. Immunocytochemistry and electron microscopy were used to characterize the intracellular localization of Tax and identify cellular factors which are the potential targets for its transcriptional activity. These studies indicated that Tax localizes in discrete nuclear foci in T lymphocytes transformed by HTLV-1 and in cells transduced with Tax expression vectors. The Tax-containing foci are complex nuclear structures comprising a central core in which Tax colocalizes with splicing factor Sm. In addition to splicing factors Sm and SC-35, the Tax-containing nuclear structures also contain transcriptional components, including the largest subunit of RNA polymerase II and cyclin-dependent kinase CDK8. The inclusion of the two subunits of NF-kappaB, p50 and RelA, and the presence of the mRNA from a gene specifically activated by Tax through NF-kappaB binding sites suggest that these unique nuclear structures participate in Tax-mediated activation of gene expression via the NF-kappaB pathway.

MeSH Terms
Animals Autoantigens/analysis COS Cells Cell Nucleus/chemistry Gene Expression Regulation Gene Products, tax/analysis Human T-lymphotropic virus 1/chemistry Humans NF-kappa B/analysis RNA Polymerase II/analysis RNA, Messenger/analysis Ribonucleoproteins, Small Nuclear Transcription Factor RelA snRNP Core Proteins
Chemicals
Autoantigens Gene Products, tax NF-kappa B RNA, Messenger Ribonucleoproteins, Small Nuclear Transcription Factor RelA snRNP Core Proteins RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bex F
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8594/9072, USA. bex@utsw.swmed.edu
McDowall A
Burny A
Gaynor R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.5.3484-3497.1997
Email
bex@utsw.swmed.edu
Published
1997-05-00
Pages
3484-97
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191495
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.71.5.3484-3497.1997 PMC Full Text View journal details
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