Rapid detection of bacterial surface proteins using an enzyme-linked immunosorbent assay system.

Journal of biochemical and biophysical methods ·Vol. 34 ·No. 1 ·1997-02-01 ·Pages 69-71

Burkovski A

Abstract

In this communication a rapid and simple enzyme-linked immunosorbent assay is described, suitable for the investigation of bacterial surface proteins. The protocol uses whole cells and avoids any centrifugation or cross-linking steps. Less than 10(6) cells were detected by this procedure.

MeSH Terms

ATP-Binding Cassette Transporters/analysis,immunology Amino Acid Transport System X-AG Antibodies, Bacterial Bacterial Proteins/analysis Colony Count, Microbial Corynebacterium/immunology,isolation & purification Enzyme-Linked Immunosorbent Assay/methods Evaluation Studies as Topic Membrane Proteins/analysis

Chemicals

ATP-Binding Cassette Transporters Amino Acid Transport System X-AG Antibodies, Bacterial Bacterial Proteins Membrane Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Burkovski A

Forschungszentrum Jülich, Institut für Biotechnologie 1, Germany. A.Burkovski@kfajuelich.de

Article Info

Journal

Journal of biochemical and biophysical methods

Abbr.

J Biochem Biophys Methods

ISSN

0165-022X

DOI

10.1016/s0165-022x(96)00034-6

a.burkovski@kfajuelich.de

Published

1997-02-01

Pages

69-71

Language

English

Region

Netherlands

NLM ID

7907378

Subset

External Links

PubMed source DOI: 10.1016/s0165-022x(96)00034-6 View journal details

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Rapid detection of bacterial surface proteins using an enzyme-linked immunosorbent assay system.

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