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PMID: 9075187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L.

Lipids ·Vol. 32 ·No. 1 ·1997-01-00 ·Pages 7-12

Ratledge C, Bowater MD, Taylor PN

Abstract

The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl-CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl-CoA formed min-1.g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo, could account for half of the acetyl-CoA required for the measured rate of fatty acid biosynthesis during seed development. The enzyme appeared to be localized in a subcellular compartment, which was clearly separated from mitochondria on a sucrose gradient and by differential centrifugation, and which corresponded to the chloroplast organelle.

MeSH Terms
ATP Citrate (pro-S)-Lyase/metabolism Acetates/metabolism Acetyl-CoA Carboxylase/metabolism Brassica/enzymology Cell Fractionation Centrifugation, Density Gradient Chloroplasts/enzymology,metabolism Citric Acid/metabolism Cytosol/enzymology,metabolism Fumarate Hydratase/metabolism Glucosephosphate Dehydrogenase/metabolism Hydrogen-Ion Concentration Lipid Metabolism Lipids/biosynthesis Malate Dehydrogenase/metabolism Mitochondria/enzymology,metabolism Plant Oils/metabolism Seeds/enzymology,metabolism
Chemicals
Acetates Lipids Plant Oils Citric Acid Malate Dehydrogenase malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) Glucosephosphate Dehydrogenase ATP Citrate (pro-S)-Lyase Fumarate Hydratase Acetyl-CoA Carboxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ratledge C
Department of Biological Sciences, University of Hull, United Kingdom.
Bowater M D
Taylor P N
References (16)
16 references, click to expand
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Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/s11745-997-0002-7
Published
1997-01-00
Pages
7-12
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/s11745-997-0002-7 View journal details
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