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PMID: 9074928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

One-megabase sequence analysis of the human immunoglobulin lambda gene locus.

Genome research ·Vol. 7 ·No. 3 ·1997-03-00 ·Pages 250-61

Kawasaki K, Minoshima S, Nakato E, Shibuya K, Shintani A, Schmeits JL, Wang J, Shimizu N

Abstract

A total of 1,025,415 bases of nucleotide sequence, including the entire human immunoglobulin lambda gene locus has been determined. This is the largest contiguous human DNA sequence ever published. The sequence data revealed the organization of 36 potentially active V lambda gene segments, 33 pseudogene segments, and seven J lambda-C lambda gene segments. Among these 69 functional or nonfunctional V lambda gene segments, 32 were newly discovered. These V lambda gene segments are located within five gene-rich clusters and are divided into five clans based on sequence identity. Five potentially active nonimmunoglobulin genes were also detected within the lambda gene locus, and two other genes were observed in the upstream region. Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V lambda gene segments.

MeSH Terms

Alleles Alternative Splicing Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Cosmids DNA/analysis DNA Mutational Analysis Exons Gene Expression Humans Immunoglobulin Constant Regions/genetics Immunoglobulin Joining Region/genetics Immunoglobulin Variable Region/genetics Immunoglobulin lambda-Chains/genetics Introns Molecular Sequence Data Multigene Family/genetics Phylogeny Pseudogenes/genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid

Chemicals

Immunoglobulin Constant Regions Immunoglobulin Joining Region Immunoglobulin Variable Region Immunoglobulin lambda-Chains DNA

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Kawasaki K

Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.

Minoshima S

Nakato E

Shibuya K

Shintani A

Schmeits J L

Wang J

Shimizu N

Article Info

Journal

Genome research

Abbr.

Genome Res

ISSN

1088-9051

DOI

10.1101/gr.7.3.250

Published

1997-03-00

Pages

250-61

Language

English

Region

United States

NLM ID

9518021

Subset

Databases

GENBANK

D86989, D86990, D86991, D86992, D86993, D86994, D86995, D86996, D86997, D86998, D86999, D87000, D87001, D87002, D87003, D87004, D87005, D87006, D87007, D87008, D87009, D87010, D87011, D87012, D87013, D87014, D87015, D87016, D87017, D87018

External Links

PubMed source DOI: 10.1101/gr.7.3.250 View journal details

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One-megabase sequence analysis of the human immunoglobulin lambda gene locus.

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