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PMID: 9069114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interval-specific congenic strains (ISCS): an experimental design for mapping a QTL into a 1-centimorgan interval.

Mammalian genome : official journal of the International Mammalian Genome Society ·Vol. 8 ·No. 3 ·1997-03-00 ·Pages 163-7

Darvasi A

Abstract

A general experimental design that allows mapping of a quantitative trait locus (QTL) into a 1-cM interval is presented. The design consists of a series of strains, termed "interval-specific congenic strains (ISCS)". Each ISCS is recombinant at a specific 1-cM sub-interval out of an ordered set of sub-intervals, which together comprise a wider interval, to which a QTL was previously mapped. It is shown that a specific and previously detected QTL of moderate or even small effect can be accurately mapped into a 1-cM interval in a program involving a total of no more than 1000 individuals. Consequently, ISCS can serve as the ultimate genetic mapping procedure before the application of physical mapping tools for positional cloning of a QTL.

MeSH Terms
Chromosome Mapping/methods Crosses, Genetic Genetic Markers Inbreeding Models, Genetic Species Specificity
Chemicals
Genetic Markers
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Darvasi A
Génétique Neurogénétique et Comportement, URA 1294 CNRS, UFR Biomédicale, Université Rene Descartes (Paris V), 45 rue des Saints-Pères, 75270 Paris Cedex 06, France.
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Article Info
Journal
Mammalian genome : official journal of the International Mammalian Genome Society
Abbr.
Mamm Genome
ISSN
0938-8990
DOI
10.1007/s003359900382
Published
1997-03-00
Pages
163-7
Language
English
Region
United States
NLM ID
9100916
Subset
IM
External Links
PubMed source DOI: 10.1007/s003359900382 View journal details
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