Home LiteratureArticle Details
PMID: 9049275 Published · ppublish English Journal Article

The beta-phaseolin 5' matrix attachment region acts as an enhancer facilitator.

Plant molecular biology ·Vol. 33 ·No. 3 ·1997-02-00 ·Pages 553-7

van der Geest AH, Hall TC

Abstract

MARs found flanking the beta-phaseolin gene (phas) were tested for insulating activity in an enhancer blocking assay. True insulators should block enhancer dependent expression of a reporter gene when placed between the enhancer and a promoter. Insertion of phas 3' MAR or coding sequences lowered CaMV 35S enhancer driven GUS expression from the phas basal promoter, indicating a distance dependence of the 35S enhancer. 5' MAR or 5' MAR core fragments could not act as independent enhancers when fused to the phas basal promoter, and did not lower expression when inserted in the enhancer blocking assay construct, indicating that they facilitated 35S enhancer expression at a distance when located between the enhancer and the promoter.

MeSH Terms
Base Sequence DNA-Binding Proteins/genetics Enhancer Elements, Genetic Fabaceae/genetics Genes, Plant Plant Proteins/genetics Plants, Genetically Modified Plants, Medicinal
Chemicals
DNA-Binding Proteins Plant Proteins phaseolin protein, Phaseolus vulgaris
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van der Geest A H
Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, College Station 77843-3155, USA.
Hall T C
References (32)
32 references, click to expand
  1. A position-effect assay for boundaries of higher order chromosomal domains.
    Cell. 1991 Mar 8;64(5):941-50 PMID: 1848159
  2. Positive and negative cis-acting DNA domains are required for spatial and temporal regulation of gene expression by a seed storage protein promoter.
    EMBO J. 1991 Jun;10(6):1469-79 PMID: 2026144
  3. SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin.
    EMBO J. 1993 Aug;12(8):3237-47 PMID: 8344261
  4. Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain.
    Mol Cell Biol. 1995 Jan;15(1):198-207 PMID: 7799927
  5. An ectopic copy of the Drosophila ftz associated SAR neither reorganizes local chromatin structure nor hinders elution of a chromatin fragment from isolated nuclei.
    EMBO J. 1991 May;10(5):1237-43 PMID: 1850697
  6. Nuclear organization: uniting replication foci, chromatin domains and chromosome structure.
    Bioessays. 1995 Jul;17(7):587-91 PMID: 7646480
  7. Sequences required for enhancer blocking activity of scs are located within two nuclease-hypersensitive regions.
    EMBO J. 1994 Dec 15;13(24):5984-93 PMID: 7813436
  8. Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice.
    EMBO J. 1990 Sep;9(9):2843-8 PMID: 2390972
  9. Properties of an isolated transcription stimulating sequence derived from the cauliflower mosaic virus 35S promoter.
    Plant Mol Biol. 1988 May;10(3):263-72 PMID: 24277520
  10. Nuclear Matrix Attachment Regions and Transgene Expression in Plants.
    Plant Physiol. 1996 Jan;110(1):15-21 PMID: 12226170
  11. Chromatin domains as potential units of eukaryotic gene function.
    Curr Opin Genet Dev. 1994 Apr;4(2):260-4 PMID: 8032204
  12. SARs stimulate but do not confer position independent gene expression.
    Nucleic Acids Res. 1994 Oct 25;22(21):4386-94 PMID: 7971269
  13. Reduced Position Effect in Mature Transgenic Plants Conferred by the Chicken Lysozyme Matrix-Associated Region.
    Plant Cell. 1994 Mar;6(3):417-426 PMID: 12244242
  14. A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer.
    Plant Mol Biol. 1996 Nov;32(4):579-88 PMID: 8980510
  15. New plant binary vectors with selectable markers located proximal to the left T-DNA border.
    Plant Mol Biol. 1992 Dec;20(6):1195-7 PMID: 1463855
  16. A group of scs elements function as domain boundaries in an enhancer-blocking assay.
    Mol Cell Biol. 1992 May;12(5):2424-31 PMID: 1569958
  17. A nuclear DNA attachment element mediates elevated and position-independent gene activity.
    Nature. 1989 Sep 28;341(6240):343-5 PMID: 2797152
  18. Visualization of chromosomal domains with boundary element-associated factor BEAF-32.
    Cell. 1995 Jun 16;81(6):879-89 PMID: 7781065
  19. Dependence of enhancer-mediated transcription of the immunoglobulin mu gene on nuclear matrix attachment regions.
    Science. 1994 Aug 26;265(5176):1221-5 PMID: 8066460
  20. Scaffold attachment regions increase reporter gene expression in stably transformed plant cells.
    Plant Cell. 1993 Jun;5(6):603-13 PMID: 8329896
  21. Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco.
    Plant Cell. 1992 Apr;4(4):463-71 PMID: 1498604
  22. Combinatorial and synergistic properties of CaMV 35S enhancer subdomains.
    EMBO J. 1990 Jun;9(6):1685-96 PMID: 2347302
  23. Variable patterns of expression of luciferase in transgenic tobacco leaves.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9183-7 PMID: 2251262
  24. Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice.
    Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6943-7 PMID: 1495984
  25. Chromatin and gene expression: constant questions, but changing answers.
    Cell. 1994 Nov 4;79(3):397-406 PMID: 7954807
  26. An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants.
    Transgenic Res. 1993 Mar;2(2):93-100 PMID: 8513340
  27. The transcription of chromatin templates.
    Curr Opin Genet Dev. 1994 Apr;4(2):245-54 PMID: 8032202
  28. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  29. The MAR-Mediated Reduction in Position Effect Can Be Uncoupled from Copy Number-Dependent Expression in Transgenic Plants.
    Plant Cell. 1995 May;7(5):599-609 PMID: 12242378
  30. A 5' element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila.
    Cell. 1993 Aug 13;74(3):505-14 PMID: 8348617
  31. The role of scaffold attachment regions in the structural and functional organization of plant chromatin.
    Transgenic Res. 1994 May;3(3):195-202 PMID: 8025597
  32. Regulation of beta-glucuronidase expression in transgenic tobacco plants by an A/T-rich, cis-acting sequence found upstream of a French bean beta-phaseolin gene.
    Plant Cell. 1989 Sep;1(9):839-53 PMID: 2535526
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005765525436
Published
1997-02-00
Pages
553-7
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1005765525436 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2