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PMID: 9022073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53.

The Journal of clinical investigation ·Vol. 99 ·No. 3 ·1997-02-01 ·Pages 403-13

Müller M, Strand S, Hug H, Heinemann EM, Walczak H, Hofmann WJ, Stremmel W, Krammer PH, Galle PR

Abstract

Chemotherapeutic drugs are cytotoxic by induction of apoptosis in drug-sensitive cells. We investigated the mechanism of bleomycin-induced cytotoxicity in hepatoma cells. At concentrations present in the sera of patients during therapy, bleomycin induced transient accumulation of nuclear wild-type (wt) p53 and upregulated expression of cell surface CD95 (APO-1/Fas) receptor in hepatoma cells carrying wt p53 (HepG2). Bleomycin did not increase CD95 in hepatoma cells with mutated p53 (Huh7) or in hepatoma cells which were p53-/- (Hep3B). In addition, sensitivity towards CD95-mediated apoptosis was also increased in wt p53 positive HepG2 cells. Microinjection of wt p53 cDNA into HepG2 cells had the same effect. In contrast, bleomycin did not enhance susceptibility towards CD95-mediated apoptosis in Huh7 and in Hep3B cells. Furthermore, bleomycin treatment of HepG2 cells increased CD95 ligand (CD95L) mRNA expression. Most notably, bleomycin-induced apoptosis in HepG2 cells was almost completely inhibited by antibodies which interfere with CD95 receptor/ligand interaction. These data suggest that apoptosis induced by bleomycin is mediated, at least in part, by p53-dependent stimulation of the CD95 receptor/ligand system. The same applies to other anti-cancer drugs such as cisplatin and methotrexate. These data may have major consequences for drug treatment of cancer and the explanation of drug sensitivity and resistance.

MeSH Terms
Antibodies, Blocking/immunology Antimetabolites, Antineoplastic/pharmacology,therapeutic use,toxicity Antineoplastic Agents/pharmacology,therapeutic use,toxicity Apoptosis/drug effects,genetics,immunology Bleomycin/pharmacology,therapeutic use,toxicity Blotting, Western Carcinoma, Hepatocellular/drug therapy Cells, Cultured Cisplatin/pharmacology,therapeutic use,toxicity Cytotoxicity Tests, Immunologic DNA Fragmentation DNA, Complementary/genetics Drug Resistance, Neoplasm Fas Ligand Protein Gene Expression Regulation, Neoplastic Genes, p53 Humans Immunohistochemistry In Situ Hybridization Membrane Glycoproteins/genetics,immunology Methotrexate/pharmacology,therapeutic use,toxicity Microinjections Polymerase Chain Reaction RNA, Messenger/analysis,biosynthesis fas Receptor/immunology
Chemicals
Antibodies, Blocking Antimetabolites, Antineoplastic Antineoplastic Agents DNA, Complementary FASLG protein, human Fas Ligand Protein Membrane Glycoproteins RNA, Messenger fas Receptor Bleomycin Cisplatin Methotrexate
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Müller M
University Hospital, Department of Internal Medicine IV, Heidelberg, Germany.
Strand S
Hug H
Heinemann E M
Walczak H
Hofmann W J
Stremmel W
Krammer P H
Galle P R
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
Published
1997-02-01
Pages
403-13
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC507813
Subset
IM
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