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PMID: 9020076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of putative progenitor endothelial cells for angiogenesis.

Science (New York, N.Y.) ·Vol. 275 ·No. 5302 ·1997-02-14 ·Pages 964-7

Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner JM

Abstract

Putative endothelial cell (EC) progenitors or angioblasts were isolated from human peripheral blood by magnetic bead selection on the basis of cell surface antigen expression. In vitro, these cells differentiated into ECs. In animal models of ischemia, heterologous, homologous, and autologous EC progenitors incorporated into sites of active angiogenesis. These findings suggest that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarian angiogenesis.

MeSH Terms

Animals Antigens, CD34/analysis Biomarkers/analysis Cell Differentiation Cell Separation Cells, Cultured Endothelium, Vascular/chemistry,cytology Flow Cytometry Hindlimb/blood supply Humans Ischemia/physiopathology Mice Mice, Inbred C57BL Mice, Nude Mice, Transgenic Neovascularization, Physiologic Nitric Oxide Synthase/analysis Rabbits Receptor Protein-Tyrosine Kinases/analysis Receptors, Growth Factor/analysis Receptors, Vascular Endothelial Growth Factor Stem Cells/chemistry,cytology

Chemicals

Antigens, CD34 Biomarkers Receptors, Growth Factor Nitric Oxide Synthase Receptor Protein-Tyrosine Kinases Receptors, Vascular Endothelial Growth Factor

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Asahara T

Department of Medicine (Cardiology), St. Elizabeth's Medical Center, Tufts University School of Medicine, 736 Cambridge Street, Boston, MA 02135, USA.

Murohara T

Sullivan A

Silver M

van der Zee R

Li T

Witzenbichler B

Schatteman G

Isner J M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.275.5302.964

Published

1997-02-14

Pages

964-7

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

PHS HHS · 2824 · United States

PHS HHS · 53354 · United States

PHS HHS · 57516 · United States

External Links

PubMed source DOI: 10.1126/science.275.5302.964 View journal details

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Isolation of putative progenitor endothelial cells for angiogenesis.

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