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PMID: 9008277 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mycobacterium tuberculosis efpA encodes an efflux protein of the QacA transporter family.

Clinical and diagnostic laboratory immunology ·Vol. 4 ·No. 1 ·1997-01-00 ·Pages 23-32

Doran JL, Pang Y, Mdluli KE, Moran AJ, Victor TC, Stokes RW, Mahenthiralingam E, Kreiswirth BN, Butt JL, Baron GS, Treit JD, Kerr VJ, Van Helden PD, Roberts MC, Nano FE

Abstract

The Mycobacterium tuberculosis H37Rv efpA gene encodes a putative efflux protein, EfpA, of 55,670 Da. The deduced EfpA protein was similar in secondary structure to Pur8, MmrA, TcmA, LfrA, EmrB, and other members of the QacA transporter family (QacA TF) which mediate antibiotic and chemical resistance in bacteria and yeast. The predicted EfpA sequence possessed all transporter motifs characteristic of the QacA TF, including those associated with proton-antiport function and the motif considered to be specific to exporters. The 1,590-bp efpA open reading frame was G+C rich (65%), whereas the 40-bp region immediately upstream had an A+T bias (35% G+C). Reverse transcriptase-PCR assays indicated that efpA was expressed in vitro and in situ. Putative promoter sequences were partially overlapped by the A+T-rich region and by a region capable of forming alternative secondary structures indicative of transcriptional regulation in analogous systems. PCR single-stranded conformational polymorphism analysis demonstrated that these upstream flanking sequences and the 231-bp, 5' coding region are highly conserved among both drug-sensitive and multiply-drug-resistant isolates of M. tuberculosis. The efpA gene was present in the slow-growing human pathogens M. tuberculosis, Mycobacterium leprae, and Mycobacterium bovis and in the opportunistic human pathogens Mycobacterium avium and Mycobacterium intracellular. However, efpA was not present in 17 other opportunistically pathogenic or nonpathogenic mycobacterial species.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,genetics,metabolism Base Sequence Biological Transport/genetics Carrier Proteins/analysis,chemistry,genetics,metabolism Cloning, Molecular Humans Membrane Transport Proteins Molecular Sequence Data Mycobacterium bovis/genetics Mycobacterium leprae/genetics Mycobacterium tuberculosis/genetics Polymerase Chain Reaction
Chemicals
Bacterial Proteins Carrier Proteins Membrane Transport Proteins qacA protein, Staphylococcus aureus
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Doran J L
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada. jdoran@uvic.ca
Pang Y
Mdluli K E
Moran A J
Victor T C
Stokes R W
Mahenthiralingam E
Kreiswirth B N
Butt J L
Baron G S
Treit J D
Kerr V J
Van Helden P D
Roberts M C
Nano F E
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Article Info
Journal
Clinical and diagnostic laboratory immunology
Abbr.
Clin Diagn Lab Immunol
ISSN
1071-412X
Published
1997-01-00
Pages
23-32
Language
English
Region
United States
NLM ID
9421292
PMCID
PMC170471
Subset
IM
Grants
NIAID NIH HHS · AI24136 · United States
Databases
GENBANK
L39922
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