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PMID: 9006060 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.

Journal of bacteriology ·Vol. 179 ·No. 3 ·1997-02-00 ·Pages 976-81

Mansilla MC, de Mendoza D

Abstract

Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cysteine and sulfide and induced by sulfur limitation, indicating that cysH is controlled at the level of transcription.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/drug effects,enzymology,genetics Base Sequence Chromosome Mapping Cloning, Molecular Cysteine/biosynthesis,pharmacology Enzyme Repression Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Mutation Recombinant Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Sulfides/pharmacology Sulfotransferases/biosynthesis,genetics
Chemicals
Recombinant Proteins Sulfides PAPS sulfotransferase Sulfotransferases Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mansilla M C
Programa Multidisciplinario de Biología Experimental (PROMUBIE-CONICET) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario,Argentina.
de Mendoza D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.3.976-981.1997
Published
1997-02-00
Pages
976-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178787
Subset
IM
Databases
GENBANK
AJ000974, U76751
External Links
PubMed source DOI: 10.1128/jb.179.3.976-981.1997 PMC Full Text View journal details
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