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PMID: 9003621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A Mycobacterium tuberculosis IS6110 preferential locus (ipl) for insertion into the genome.

Journal of clinical microbiology ·Vol. 35 ·No. 2 ·1997-02-00 ·Pages 479-81

Fang Z, Forbes KJ

Abstract

A 267-nucleotide Mycobacterium tuberculosis genomic sequence (ipl, the IS6110 preferential locus) which can harbor the insertion sequence IS6110 at six alternative locations has been identified in some three-quarters of the isolates tested. Only one IS6110 copy was observed at this locus in the ipl::IS6110(+)-containing isolates tested, and all insertions had the same orientation. The implications of this finding for IS6110 fingerprint typing methods is discussed in this work.

MeSH Terms

Base Sequence DNA Fingerprinting DNA Primers DNA Transposable Elements Genome, Bacterial Humans Molecular Sequence Data Mycobacterium tuberculosis/genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length

Chemicals

DNA Primers DNA Transposable Elements

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fang Z

Medical Microbiology, Aberdeen University, Foresterhill, Aberdeen, United Kingdom.

Forbes K J

References (11)

11 references, click to expand

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IS6110, an IS-like element of Mycobacterium tuberculosis complex.
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Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.
J Clin Microbiol. 1990 Sep;28(9):2051-8 PMID: 1977765
Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family.
Mol Microbiol. 1990 Sep;4(9):1607-13 PMID: 1981088
Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains.
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An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae.
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DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology.
J Clin Microbiol. 1992 Jun;30(6):1551-6 PMID: 1352518
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J Clin Microbiol. 1993 Feb;31(2):406-9 PMID: 8381814
Analysis of the regions responsible for IS6110 RFLP in a single Mycobacterium tuberculosis strain.
Res Microbiol. 1992 Oct;143(8):767-72 PMID: 1363676
Population genetics of Mycobacterium tuberculosis complex in Scotland analysed by pulsed-field gel electrophoresis.
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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.35.2.479-481.1997

Published

1997-02-00

Pages

479-81

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC229605

Subset

Databases

GENBANK

X95799, X98148, X98149, X98150, X98151, X98152, X98153, X98154, X98155, X98156, X98157, X98158, Y13470, Y14613, Y14614

External Links

PubMed source DOI: 10.1128/jcm.35.2.479-481.1997 PMC Full Text View journal details

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A Mycobacterium tuberculosis IS6110 preferential locus (ipl) for insertion into the genome.

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