Characterization of the WIDR: a human colon carcinoma cell line.

In vitro ·Vol. 15 ·No. 6 ·1979-06-00 ·Pages 401-8

Noguchi P, Wallace R, Johnson J, Earley EM, O'Brien S, Ferrone S, Pellegrino MA, Milstien J, Needy C, Browne W, Petricciani J

Abstract

We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally, it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model cell line for tumor cell biology investigations.

MeSH Terms

Carcinoembryonic Antigen/analysis Cell Division Cell Line Colonic Neoplasms/pathology HLA Antigens Humans Isoenzymes/analysis Karyotyping Neoplasms, Experimental/etiology RNA-Directed DNA Polymerase/metabolism

Chemicals

Carcinoembryonic Antigen HLA Antigens Isoenzymes RNA-Directed DNA Polymerase

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Noguchi P

Wallace R

Johnson J

Earley E M

O'Brien S

Ferrone S

Pellegrino M A

Milstien J

Needy C

Browne W

Petricciani J

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Article Info

Journal

In vitro

Abbr.

In Vitro

ISSN

0073-5655

DOI

10.1007/BF02618407

Published

1979-06-00

Pages

401-8

Language

English

Region

United States

NLM ID

0063733

Subset

External Links

PubMed source DOI: 10.1007/BF02618407

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Characterization of the WIDR: a human colon carcinoma cell line.

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