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PMID: 8991092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

AP-2-containing clathrin coats assemble on mature lysosomes.

The Journal of cell biology ·Vol. 135 ·No. 6 Pt 2 ·1996-12-00 ·Pages 1801-14

Traub LM, Bannykh SI, Rodel JE, Aridor M, Balch WE, Kornfeld S

Abstract

Coat proteins appear to play a general role in intracellular protein trafficking by coordinating a membrane budding event with cargo selection. Here we show that the AP-2 adaptor, a clathrin-associated coat-protein complex that nucleates clathrin-coated vesicle formation at the cell surface, can also initiate the assembly of normal polyhedral clathrin coats on dense lysosomes under physiological conditions in vitro. Clathrin coat formation on lysosomes is temperature dependent, displays an absolute requirement for ATP, and occurs in both semi-intact cells and on purified lysosomes, suggesting that clathrin-coated vesicles might regulate retrograde membrane traffic out of the lysosomal compartment.

MeSH Terms
Animals Antigens, CD/analysis,metabolism Brain/cytology Cell Membrane Permeability Cells, Cultured/chemistry,metabolism,ultrastructure Clathrin/analysis Coated Vesicles/chemistry,ultrastructure DNA-Binding Proteins/analysis,metabolism Fluorescent Antibody Technique Freeze Etching Kidney/cytology Liver/cytology Lysosome-Associated Membrane Glycoproteins Lysosomes/chemistry,metabolism,ultrastructure Membrane Glycoproteins/analysis,metabolism Membrane Proteins/analysis Microscopy, Electron Rats Transcription Factor AP-2 Transcription Factors/analysis,metabolism
Chemicals
Antigens, CD Clathrin DNA-Binding Proteins Lysosome-Associated Membrane Glycoproteins Membrane Glycoproteins Membrane Proteins Transcription Factor AP-2 Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Traub L M
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Bannykh S I
Rodel J E
Aridor M
Balch W E
Kornfeld S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.135.6.1801
Published
1996-12-00
Pages
1801-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133961
Subset
IM
Grants
NCI NIH HHS · CA 08759 · United States
NCI NIH HHS · CA 586689 · United States
External Links
PubMed source DOI: 10.1083/jcb.135.6.1801 PMC Full Text View journal details
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