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PMID: 8990170 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo analysis of the functional domains of the Drosophila splicing regulator RBP1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 1 ·1997-01-07 ·Pages 115-20

Heinrichs V, Baker BS

Abstract

The Drosophila splicing factor RBP1 participates together with TRA and TRA-2 in the regulation of alternative splicing of doublesex (dsx) pre-mRNA. It does so by recognizing RBP1 RNA target sequences in the dsx pre-mRNA. RBP1 belongs to the Ser-Arg-rich (SR) protein family of splicing factors, which have in common a N-terminal RNA recognition motif-type RNA binding domain, a Gly-rich region, and a C-terminal SR domain. Using a tissue culture transfection assay, we demonstrate that the Gly residues within the Gly-rich domain, the ribonucleoprotein motifs within the RNA recognition motif RNA binding domain, and the SR domain are required for regulation of dsx splicing by RBP1 in vivo. Furthermore, using a two-hybrid system, we show protein-protein interactions between RBP1 and itself and between RBP1 and TRA-2. The SR domain and the Gly residues within the Gly-rich domain of RBP1 were found to be involved in these protein-protein interactions. Our results suggest that RBP1 and TRA-2 function in regulation of dsx splicing by forming a complex.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Base Sequence Binding Sites Culture Techniques DNA Mutational Analysis DNA-Binding Proteins/genetics Drosophila/genetics Drosophila Proteins Insect Hormones/genetics Molecular Sequence Data Protein Binding RNA Splicing Factors RNA-Binding Proteins/genetics,metabolism Ribonucleoproteins/metabolism Sequence Deletion Structure-Activity Relationship Transfection
Chemicals
DNA-Binding Proteins DSX protein, Drosophila Drosophila Proteins Insect Hormones RBP1 protein, Drosophila RNA Splicing Factors RNA-Binding Proteins Ribonucleoproteins tra2 protein, Drosophila
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heinrichs V
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
Baker B S
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.1.115
Published
1997-01-07
Pages
115-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19250
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.1.115 PMC Full Text View journal details
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