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PMID: 8989877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sink-specific and stress-regulated Arabidopsis STP4 gene: enhanced expression of a gene encoding a monosaccharide transporter by wounding, elicitors, and pathogen challenge.

The Plant cell ·Vol. 8 ·No. 12 ·1996-12-00 ·Pages 2169-82

Truernit E, Schmid J, Epple P, Illig J, Sauer N

Abstract

A cDNA for the Arabidopsis STP4 gene (for sugar transport protein 4) was isolated, and the properties of the encoded protein were studied in Schizosaccharomyces pombe. The STP4 monosaccharide H+ symporter is composed of 514 amino acids and has a calculated molecular mass of 57.1 kD. RNA gel blot analyses revealed that STP4 is expressed primarily in roots and flowers of Arabidopsis. This was shown in more detail with STP4 promoter-beta-glucuronidase (GUS) plants yielding strong STP4-driven GUS activity in root tips and anthers. Wounding of plants transformed with STP4-GUS constructs resulted in a rapid increase in GUS activity in cells directly adjacent to the lesion. This was confirmed by RNase protection analyses in Arabidopsis wild-type plants showing a strong, wound-induced increase in STP4 mRNA levels. STP4 expression was induced rapidly in suspension-cultured Arabidopsis cells that were treated with the Pseudomonas syringae elicitor or with chitin or in Arabidopsis plants that were exposed to fungal attacks. Our data suggest that the role of STP4 is to catalyze monosaccharide import into classic sinks, such as root tips and anthers, and, most importantly, to meet the increased carbohydrate demand of cells responding to environmental stress.

MeSH Terms
3-O-Methylglucose/metabolism Amino Acid Sequence Arabidopsis/genetics,microbiology,physiology Arabidopsis Proteins Base Sequence Cloning, Molecular DNA, Complementary Gene Expression Regulation, Plant Genes, Plant Glucuronidase/biosynthesis Molecular Sequence Data Monosaccharide Transport Proteins/biosynthesis,chemistry,genetics Monosaccharides/metabolism Plant Roots Plants, Genetically Modified Plants, Toxic Pseudomonas/pathogenicity Recombinant Fusion Proteins/biosynthesis Restriction Mapping Schizosaccharomyces Tobacco/metabolism Wounds and Injuries
Chemicals
Arabidopsis Proteins DNA, Complementary Monosaccharide Transport Proteins Monosaccharides Recombinant Fusion Proteins STP4 protein, Arabidopsis 3-O-Methylglucose Glucuronidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Truernit E
Lehrstuhl Botanik II, Universität Erlangen-Nürnberg, Germany.
Schmid J
Epple P
Illig J
Sauer N
References (40)
40 references, click to expand
  1. Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1408-12 PMID: 2937058
  2. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  3. Chitin oligosaccharides as elicitors of chitinase activity in melon plants.
    Biochem Biophys Res Commun. 1987 Mar 30;143(3):885-92 PMID: 3566760
  4. Plant Defense Response to Fungal Pathogens (Activation of Host-Plasma Membrane H+-ATPase by Elicitor-Induced Enzyme Dephosphorylation).
    Plant Physiol. 1994 Jan;104(1):209-215 PMID: 12232073
  5. Nucleotide sequence of the 18S rRNA gene from Arabidopsis thaliana Co10.
    Nucleic Acids Res. 1989 Sep 25;17(18):7513 PMID: 2798101
  6. Comparison of proteinase inhibitor-inducing activities and phytoalexin elicitor activities of a pure fungal endopolygalacturonase, pectic fragments, and chitosans.
    Plant Physiol. 1984 Nov;76(3):833-6 PMID: 16663934
  7. Octopine Ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the T-region.
    Plasmid. 1982 Jan;7(1):15-29 PMID: 6283573
  8. Topology of the Glut 1 glucose transporter deduced from glycosylation scanning mutagenesis.
    J Biol Chem. 1994 Aug 12;269(32):20482-8 PMID: 8051147
  9. Functional reconstitution of the solubilized Arabidopsis thaliana STP1 monosaccharide-H+ symporter in lipid vesicles and purification of the histidine tagged protein from transgenic Saccharomyces cerevisiae.
    Plant J. 1994 Aug;6(2):225-33 PMID: 7920712
  10. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.
    Gene. 1984 Dec;32(3):481-5 PMID: 6099327
  11. The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe.
    J Biol Chem. 1983 Jan 10;258(1):143-9 PMID: 6294096
  12. Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana.
    EMBO J. 1990 Oct;9(10):3045-50 PMID: 2209537
  13. Nystatin changes the properties of transporters for arginine and sugars. An in vitro study.
    FEBS Lett. 1994 Aug 15;350(1):46-50 PMID: 8062922
  14. Mechanisms of induced resistance in plants.
    Annu Rev Microbiol. 1983;37:51-79 PMID: 6357059
  15. Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection.
    J Exp Med. 1990 Aug 1;172(2):657-60 PMID: 2373996
  16. A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport.
    Trends Biochem Sci. 1993 Jan;18(1):13-20 PMID: 8438231
  17. The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.
    FEBS Lett. 1989 Dec 18;259(1):43-6 PMID: 2599110
  18. Glucose-transport-deficient mutants of Schizosaccharomyces pombe: phenotype, genetics and use for genetic complementation.
    Microbiology. 1994 Oct;140 ( Pt 10):2617-23 PMID: 8000531
  19. Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants.
    Mol Cell Biol. 1987 Dec;7(12):4337-44 PMID: 3437892
  20. Nonvascular, Symplasmic Diffusion of Sucrose Cannot Satisfy the Carbon Demands of Growth in the Primary Root Tip of Zea mays L.
    Plant Physiol. 1994 May;105(1):19-33 PMID: 12232183
  21. Subcellular localization of the inducible Chlorella HUP1 monosaccharide-H+ symporter and cloning of a Co-induced galactose-H+ symporter.
    Plant Physiol. 1995 Jan;107(1):33-41 PMID: 7870840
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrmA mutations.
    Mol Microbiol. 1996 Feb;19(4):715-28 PMID: 8820642
  24. Proton-coupled hexose transport in Chlorella vulgaris.
    FEBS Lett. 1973 Dec 15;38(1):16-8 PMID: 4772688
  25. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  26. cDNA cloning of carrot extracellular beta-fructosidase and its expression in response to wounding and bacterial infection.
    Plant Cell. 1990 Nov;2(11):1107-19 PMID: 2152110
  27. Uptake of 3-o-Methylglucose by Healthy and Hypomyces-infected Squash Hypocotyls.
    Plant Physiol. 1969 Sep;44(9):1267-72 PMID: 16657201
  28. The promoter of the Arabidopsis thaliana SUC2 sucrose-H+ symporter gene directs expression of beta-glucuronidase to the phloem: evidence for phloem loading and unloading by SUC2.
    Planta. 1995;196(3):564-70 PMID: 7647685
  29. Phloem Loading by the PmSUC2 Sucrose Carrier from Plantago major Occurs into Companion Cells.
    Plant Cell. 1995 Oct;7(10):1545-1554 PMID: 12242355
  30. Sink Metabolism in Tomato Fruit : II. Phloem Unloading and Sugar Uptake.
    Plant Physiol. 1988 Jul;87(3):731-6 PMID: 16666216
  31. Storage of competent cells for Agrobacterium transformation.
    Nucleic Acids Res. 1988 Oct 25;16(20):9877 PMID: 3186459
  32. Expression of the PmSUC1 sucrose carrier gene from Plantago major L. is induced during seed development.
    Plant J. 1996 Jan;9(1):93-100 PMID: 8580974
  33. Membrane transport proteins: implications of sequence comparisons.
    Curr Opin Cell Biol. 1992 Aug;4(4):684-95 PMID: 1419050
  34. Sequence and structure of a human glucose transporter.
    Science. 1985 Sep 6;229(4717):941-5 PMID: 3839598
  35. Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):7949-52 PMID: 11607110
  36. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  37. Expression of a sugar-transporter gene family in a photoautotrophic suspension culture of Chenopodium rubrum L.
    Planta. 1994;193(3):365-71 PMID: 7764872
  38. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536-40 PMID: 16593964
  39. cdc25+ functions as an inducer in the mitotic control of fission yeast.
    Cell. 1986 Apr 11;45(1):145-53 PMID: 3955656
  40. Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases.
    Nature. 1986 Feb 20-26;319(6055):689-93 PMID: 3005867
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.8.12.2169
Published
1996-12-00
Pages
2169-82
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161343
Subset
IM
Databases
GENBANK
X66857
External Links
PubMed source DOI: 10.1105/tpc.8.12.2169 PMC Full Text View journal details
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