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PMID: 8986840 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 26 ·1996-12-24 ·Pages 15497-502

Leister RT, Ausubel FM, Katagiri F

Abstract

The Arabidopsis thaliana disease resistance genes RPS2 and RPM1 belong to a class of plant disease resistance genes that encode proteins that contain an N-terminal tripartite nucleotide binding site (NBS) and a C-terminal tandem array of leucine-rich repeats. RPS2 and RPM1 confer resistance to strains of the bacterial phytopathogen Pseudomonas syringae carrying the avirulence genes avrRpt2 and avrB, respectively. In these gene-for-gene relationships, it has been proposed that pathogen avirulence genes generate specific ligands that are recognized by cognate receptors encoded by the corresponding plant resistance genes. To test this hypothesis, it is crucial to know the site of the potential molecular recognition. Mutational analysis of RPS2 protein and in vitro translation/translocation studies indicated that RPS2 protein is localized in the plant cytoplasm. To determine whether avirulence gene products themselves are the ligands for resistance proteins, we expressed the avrRpt2 and avrB genes directly in plant cell using a novel quantitative transient expression assay, and found that expression of avrRpt2 and avrB elicited a resistance response in plants carrying the corresponding resistance genes. This observation indicates that no bacterial factors other than the avirulence gene products are required for the specific resistance response as long as the avirulence gene products are correctly localized. We propose that molecular recognition of P. syringae in RPS2- and RPM1-specified resistance occurs inside of plant cells.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,microbiology,physiology Arabidopsis Proteins Base Sequence Cytoplasm/metabolism DNA Primers Genes, Plant Immunity, Innate Leucine Zippers Molecular Sequence Data Mutagenesis, Site-Directed Plant Diseases Plant Proteins/biosynthesis,chemistry,genetics Polymerase Chain Reaction Pseudomonas/genetics,pathogenicity Sequence Homology, Amino Acid Virulence
Chemicals
Arabidopsis Proteins DNA Primers Plant Proteins RPM1 protein, Arabidopsis RPS2 protein, Arabidopsis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leister R T
Department of Biological Sciences, University of Maryland, Baltimore 21250, USA.
Ausubel F M
Katagiri F
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.26.15497
Published
1996-12-24
Pages
15497-502
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26433
Subset
IM
Grants
PHS HHS · 48707 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.26.15497 PMC Full Text View journal details
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