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PMID: 8977340 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Antiproliferative effects of interferon alpha on human pancreatic carcinoma cell lines are associated with differential regulation of protein kinase C isoenzymes.

Gut ·Vol. 39 ·No. 2 ·1996-08-00 ·Pages 255-61

Rosewicz S, Weder M, Kaiser A, Riecken EO

Abstract

The molecular mechanisms mediating the antiproliferative effects of interferon alpha on human pancreatic carcinoma cells are poorly understood. To characterise the effects of interferon alpha on protein kinase C isoenzyme expression in interferon alpha sensitive and resistant human pancreatic tumour cell lines. The ductal human pancreatic carcinoma cell lines Capan 1 and Capan 2 were investigated. Anchorage dependent and independent growth was determined by cell number and a human tumour clonogenic assay. Interferon alpha receptor expression was examined by reverse-transcriptase polymerase chain reaction and electrophoretic mobility shift assay. Protein kinase C isoenzyme expression was evaluated by western blotting using monospecific polyclonal antibodies. Interferon alpha treatment results in a time and dose dependent inhibition of anchorage dependent and independent growth in Capan 1 cells while Capan 2 cells were not affected by interferon alpha. Both cell lines express interferon alpha receptor mRNA transcripts. Growth inhibition by interferon alpha in Capan 1 cells was paralleled by a profound decrease of protein kinase C alpha and zeta expression while these isoenzymes were unaffected in the interferon resistant cell line Capan 2. Inhibition of protein kinase C isoenzyme expression might determine the sensitivity of a given pancreatic carcinoma to respond to the antiproliferative action of interferon alpha.

MeSH Terms

Blotting, Western Carcinoma, Ductal, Breast/enzymology Cell Division Electrophoresis Humans Interferon-alpha/pharmacology Isoenzymes/antagonists & inhibitors,drug effects Pancreatic Neoplasms/enzymology Polymerase Chain Reaction Protein Kinase C/antagonists & inhibitors,drug effects Tumor Cells, Cultured/cytology,drug effects,enzymology

Chemicals

Interferon-alpha Isoenzymes Protein Kinase C

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rosewicz S

Department of Gastroenterology, Medizinische Klinik and Poliklinik, Khnikum Benjamin Franklin, Berlin, Germany.

Weder M

Kaiser A

Riecken E O

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Article Info

Journal

Gut

Abbr.

Gut

ISSN

0017-5749

DOI

10.1136/gut.39.2.255

Published

1996-08-00

Pages

255-61

Language

English

Region

England

NLM ID

2985108R

PMCID

PMC1383308

Subset

External Links

PubMed source DOI: 10.1136/gut.39.2.255 PMC Full Text View journal details

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Antiproliferative effects of interferon alpha on human pancreatic carcinoma cell lines are associated with differential regulation of protein kinase C isoenzymes.

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