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PMID: 8969508 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.

Microbiology (Reading, England) ·Vol. 142 ( Pt 11) ·1996-11-00 ·Pages 3103-11

Mizuno M, Masuda S, Takemaru K, Hosono S, Sato T, Takeuchi M, Kobayashi Y

Abstract

As part of the Bacillus subtilis genome sequencing project, we have determined a 283 kb contiguous sequence from 210 degrees to 232 degrees of the B. subtilis genome. This region contains the 48 kb skin element which is excised during sporulation by a site-specific recombinase. In this region, 310 complete ORFs and one tRNA gene were identified: 66 ORFs have been sequenced and characterized previously by other workers, e.g. acc, ans, bfm, blt, bmr, comE, comG, dnaK, rpoD and sin operons; cwiA, gpr and lysA genes; many sporulation genes and operons, spo0A, spoIIA, spoIIM, spoiiP, spoIIIA, spoIIIC, spoIVB, spoIVCA, spoIVCB and spoVA, etc. The products of 84 ORFs were found to display significant similarity to proteins with known function in data banks, e.g., proteins involved in nucleotide metabolism, lipid biosynthesis, amino acid transport (ABC transporter), phosphate-specific transport, the glycine cleavage system, the two-component regulatory system, cell wall autolysis, ferric uptake and sporulation. However, the functions of more than half of the ORFs (52%, 160 ORFs) are still unknown. In the skin element containing 60 ORFs, 32 ORFs (53%) encode proteins which have significant homology to gene products of the B. subtilis temperate phage phi 105 and/or the defective phage PBSX.

MeSH Terms

Bacillus subtilis/genetics,physiology Bacterial Proteins/genetics Chromosome Mapping Chromosomes, Bacterial/genetics Cloning, Molecular Genes, Bacterial Genome, Bacterial Molecular Sequence Data Open Reading Frames Sequence Homology, Amino Acid Spores, Bacterial/genetics,physiology

Chemicals

Bacterial Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Mizuno M

Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.

Masuda S

Takemaru K

Hosono S

Sato T

Takeuchi M

Kobayashi Y

Article Info

Journal

Microbiology (Reading, England)

Abbr.

Microbiology (Reading)

ISSN

1350-0872

DOI

10.1099/13500872-142-11-3103

Published

1996-11-00

Pages

3103-11

Language

English

Region

England

NLM ID

9430468

Subset

Databases

GENBANK

D84432, M86824, X56679

SWISSPROT

P02375, P05848, P07682, P12013, P13255, P13772, P15770, P16083, P16177, P21865, P21866, P23829, P23896, P24805, P24878, P28637, P29942, P31065, P31166, P31544, P32131, P32683, P32819, P36718, P37214, P37912, P41028

External Links

PubMed source DOI: 10.1099/13500872-142-11-3103 View journal details

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Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.

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