Home LiteratureArticle Details
PMID: 896464 Published · ppublish English Journal Article

A reexamination of the problem of resonance energy transfer between DNA intercalated chromophores using bisintercalating compounds.

Nucleic acids research ·Vol. 4 ·No. 5 ·1977-00-00 ·Pages 1361-79

Le Bret M, Le Pecq JB, Barbet J, Roques BP

Abstract

The rate of energy transfer between DNA intercalated ethidium cations calculated by Paoletti and Le Pecq1 using the Forster theory differs from the measured one by a factor of twenty two, if the proper geometrical factors are taken into account. By changing some of the parameters used in the calculation, the discrepancy can be reduced but not eliminated. This led us to the study of other systems where experimental and calculated results can be more directly compared. The apparent rate of energy transfer between ethidium and one of its non fluorescent analogues and between various pairs of intercalated chromophores has been studied. The fluorescence anisotropy decay of acridine dimers in glycerol or bisintercalated in DNA has been measured. These studies show that the Forster theory of energy transfer does not apply to the case of identical chromophores when they are relatively close to each other.

MeSH Terms
Acridines Binding Sites Circular Dichroism DNA Ellipticines/analogs & derivatives Energy Transfer Ethidium/analogs & derivatives Mathematics Nitro Compounds Nucleic Acid Conformation Polydeoxyribonucleotides Quantum Theory Spectrometry, Fluorescence Spectrophotometry
Chemicals
Acridines Ellipticines Nitro Compounds Polydeoxyribonucleotides DNA Ethidium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Le Bret M
Le Pecq J B
Barbet J
Roques B P
References (21)
21 references, click to expand
  1. Evidence for sequence preferences in the intercalative binding of ethidium bromide to dinucleoside monophosphates.
    J Mol Biol. 1975 Sep 15;97(2):133-62 PMID: 1177318
  2. The fluorescence anisotropy decay due to energy transfers occuring in the ethidium bromide-DNA complex. Determination of the deformation angle of the DNA helix.
    Biophys Chem. 1974 Apr;1(4):266-78 PMID: 4472369
  3. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.
    J Mol Biol. 1968 Apr 14;33(1):141-71 PMID: 4296517
  4. The flow birefringence of persistence length deoxyribonucleic acid. Hydrodynamic properties, optical anisotropy, and hydration shell anistropy.
    J Am Chem Soc. 1970 Nov 18;92(23):6957-64 PMID: 5483071
  5. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.
    J Mol Biol. 1974 Nov 15;89(4):783-801 PMID: 4449133
  6. DNA polyintercalating drugs: DNA binding of diacridine derivatives.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2915-9 PMID: 127172
  7. X-ray crystallographic visualization of drug-nucleic acid intercalative binding: structure of an ethidium-dinucleoside monophosphate crystalline complex, Ethidium: 5-iodouridylyl (3'-5') adenosine.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):628-32 PMID: 1054844
  8. The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium.
    J Mol Biol. 1975 Jan 5;91(1):1-13 PMID: 1102694
  9. Dependence of the kinetics of singlet-singlet energy transfer on spectral overlap.
    Proc Natl Acad Sci U S A. 1969 May;63(1):23-30 PMID: 16591747
  10. Failure of Energy Transfer between Identical Aromatic Molecules on Excitation at the Long Wave Edge of the Absorption Spectrum.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):823-30 PMID: 16591825
  11. ENERGY TRANSFER MECHANISMS AND THE MOLECULAR EXCITON MODEL FOR MOLECULAR AGGREGATES.
    Radiat Res. 1963 Sep;20:55-70 PMID: 14061481
  12. A new antitumoral agent: 9-hydroxyellipticine. Possibility of a rational design of anticancerous drugs in the series of DNA intercalating drugs.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):5078-82 PMID: 4531039
  13. Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA.
    J Mol Biol. 1970 Feb 14;47(3):419-35 PMID: 4313852
  14. Nanosecond fluorescence spectroscopy of macromolecules.
    Methods Enzymol. 1972;26:498-578 PMID: 4680723
  15. ENERGY TRANSFER. A SYSTEM WITH RELATIVELY FIXED DONOR-ACCEPTOR SEPARATION.
    J Am Chem Soc. 1965 Mar 5;87:995-1003 PMID: 14284634
  16. Optical anisotropy of synthetic polynucleotides. II. Calculation of pi-electron polarizability of uracil and adenine.
    Biopolymers. 1969 Aug;8(2):199-216 PMID: 5823500
  17. Electronic structure of ethidium bromide.
    FEBS Lett. 1973 Feb 1;29(3):227-230 PMID: 11946919
  18. Resonance energy transfer between ethidium bromide molecules bound to nucleic acids. Does intercalation wind or unwind the DNA helix?
    J Mol Biol. 1971 Jul 14;59(1):43-62 PMID: 5283756
  19. Energy transfer: a spectroscopic ruler.
    Proc Natl Acad Sci U S A. 1967 Aug;58(2):719-26 PMID: 5233469
  20. Calculation of binding isotherms for heterogenous polymers.
    Biopolymers. 1968 Apr;6(4):575-84 PMID: 5644787
  21. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.
    J Mol Biol. 1967 Jul 14;27(1):87-106 PMID: 6033613
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.5.1361
Published
1977-00-00
Pages
1361-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343761
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.5.1361 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2