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PMID: 8955308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family.

Journal of bacteriology ·Vol. 178 ·No. 23 ·1996-12-00 ·Pages 6865-72

Braun EL, Fuge EK, Padilla PA, Werner-Washburne M

Abstract

The regulation of cellular growth and proliferation in response to environmental cues is critical for development and the maintenance of viability in all organisms. In unicellular organisms, such as the budding yeast Saccharomyces cerevisiae, growth and proliferation are regulated by nutrient availability. We have described changes in the pattern of protein synthesis during the growth of S. cerevisiae cells to stationary phase (E. K. Fuge, E. L. Braun, and M. Werner-Washburne, J. Bacteriol. 176:5802-5813, 1994) and noted a protein, which we designated Snz1p (p35), that shows increased synthesis after entry into stationary phase. We report here the identification of the SNZ1 gene, which encodes this protein. We detected increased SNZ1 mRNA accumulation almost 2 days after glucose exhaustion, significantly later than that of mRNAs encoded by other postexponential genes. SNZ1-related sequences were detected in phylogenetically diverse organisms by sequence comparisons and low-stringency hybridization. Multiple SNZ1-related sequences were detected in some organisms, including S. cerevisiae. Snz1p was found to be among the most evolutionarily conserved proteins currently identified, indicating that we have identified a novel, highly conserved protein involved in growth arrest in S. cerevisiae. The broad phylogenetic distribution, the regulation of the SNZ1 mRNA and protein in S. cerevisiae, and identification of a Snz protein modified during sporulation in the gram-positive bacterium Bacillus subtilis support the hypothesis that Snz proteins are part of an ancient response that occurs during nutrient limitation and growth arrest.

MeSH Terms
Amino Acid Sequence Cell Division Cloning, Molecular Conserved Sequence DNA Probes Fungal Proteins/chemistry,genetics Genes, Bacterial Genes, Fungal Genes, Plant Molecular Sequence Data Multigene Family Mutation Phenotype RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins
Chemicals
DNA Probes Fungal Proteins RNA, Fungal RNA, Messenger SNZ1 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Braun E L
Department of Biology, University of New Mexico, Albuquerque 87131, USA.
Fuge E K
Padilla P A
Werner-Washburne M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.23.6865-6872.1996
Published
1996-12-00
Pages
6865-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178587
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.23.6865-6872.1996 PMC Full Text View journal details
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