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PMID: 8932397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An efficient DDAB-mediated transfection of Drosophila S2 cells.

Nucleic acids research ·Vol. 24 ·No. 21 ·1996-11-01 ·Pages 4362-3

Han K

Abstract

I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.

MeSH Terms

Animals Cell Line Drosophila Genes, Reporter Lac Operon Quaternary Ammonium Compounds/metabolism Transfection

Chemicals

Quaternary Ammonium Compounds didodecyldimethylammonium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Han K

Department of Genetic Engineering, Hallym University, Chunchon, Republic of Korea. khhan@sun.hallym.ac.kr

References (7)

7 references, click to expand

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A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.
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Cell lines derived from late embryonic stages of Drosophila melanogaster.
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Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
Cell. 1988 Dec 2;55(5):887-98 PMID: 3142690
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Proc Natl Acad Sci U S A. 1983 Dec;80(23):7095-8 PMID: 6417663
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/24.21.4362

khhan@sun.hallym.ac.kr

Published

1996-11-01

Pages

4362-3

Language

English

Region

England

NLM ID

0411011

PMCID

PMC146234

Subset

External Links

PubMed source DOI: 10.1093/nar/24.21.4362 PMC Full Text View journal details

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An efficient DDAB-mediated transfection of Drosophila S2 cells.

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