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PMID: 8910003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human intestinal cell lines Caco-2 and LS174T as models to study cell-type specific mucin expression.

Glycoconjugate journal ·Vol. 13 ·No. 5 ·1996-10-00 ·Pages 757-68

van Klinken BJ, Oussoren E, Weenink JJ, Strous GJ, Büller HA, Dekker J, Einerhand AW

Abstract

Mucin expression was studied during proliferation and differentiation of the enterocyte-like Caco-2 and goblet cell-like LS174T cell lines. Caco-2 cells express mRNAs of MUC1, MUC3, MUC4 and MUC5A/C whereas MUC2 and MUC6 mRNAs are virtually absent. Furthermore, MUC3 mRNA is expressed in a differentiation dependent manner, as is the case for enterocytes. Concomitantly MUC3 protein precursor (approximately 550 kDa) was detected in Caco-2 cells. In LS174T cells mucin mRNAs of MUC1, MUC2 and MUC6 are constitutively expressed at high levels, whereas MUC3, MUC4 and MUC5A/C mRNAs are present at low levels. At the protein level LS174T cells express the goblet cell specific mucin protein precursors MUC2, MUC5A/C and MUC6 with apparent molecular masses of about 600 kDa, 470/500 kDa and 400 kDa respectively. MUC3 protein is not detectable. Furthermore, human gallbladder mucin protein (approximately 470 kDa precursor), of which the gene has not yet been identified, is expressed in LS174T cells. In addition, synthesis and secretion of the goblet cell specific mature MUC2, MUC5A/C and human gallbladder mucin was demonstrated in LS174T cells. It is concluded that Caco-2 and LS174T cell lines provide excellent in vitro models to elucidate the cell-type specific mechanisms responsible for mucin expression.

MeSH Terms
Caco-2 Cells Cell Differentiation/physiology Colonic Neoplasms/metabolism DNA Primers Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Neoplastic/genetics Hexosaminidases/metabolism Humans Mucins/biosynthesis,metabolism Precipitin Tests Protein Precursors/metabolism RNA, Messenger/metabolism Tumor Cells, Cultured
Chemicals
DNA Primers Mucins Protein Precursors RNA, Messenger Hexosaminidases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van Klinken B J
Academic Medical Center, Amsterdam, The Netherlands.
Oussoren E
Weenink J J
Strous G J
Büller H A
Dekker J
Einerhand A W
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Article Info
Journal
Glycoconjugate journal
Abbr.
Glycoconj J
ISSN
0282-0080
Published
1996-10-00
Pages
757-68
Language
English
Region
United States
NLM ID
8603310
Subset
IM
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