Bacteriophage T4 middle transcription system: T4-modified RNA polymerase; AsiA, a sigma 70 binding protein; and transcriptional activator MotA.

Bacterial Proteins/isolation & purification,metabolism Bacteriophage T4/genetics,metabolism Chromatography, Affinity/methods Chromatography, DEAE-Cellulose/methods Chromatography, Gel/methods Chromatography, Ion Exchange/methods DNA/metabolism DNA-Binding Proteins/metabolism DNA-Directed RNA Polymerases/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel/methods Escherichia coli/genetics,metabolism Indicators and Reagents Phosphorus Radioisotopes Radioisotope Dilution Technique Sigma Factor/isolation & purification,metabolism Templates, Genetic Trans-Activators/isolation & purification,metabolism Transcription, Genetic Uridine Triphosphate/metabolism Viral Proteins/isolation & purification,metabolism

AsiA protein, Enterobacteria phage T4 Bacterial Proteins DNA-Binding Proteins Indicators and Reagents MotA protein, Bacteria Phosphorus Radioisotopes Sigma Factor Trans-Activators Viral Proteins DNA RNA polymerase sigma 70 DNA-Directed RNA Polymerases Uridine Triphosphate