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PMID: 8901493 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Review

The yeast, Saccharomyces cerevisiae, RNase P/MRP ribonucleoprotein endoribonuclease family.

Molecular biology reports ·Vol. 22 ·No. 2-3 ·1995-00-00 ·Pages 87-93

Reilly TH, Schmitt ME

Abstract

Ribonuclease P (RNase P) is a ribonucleoprotein responsible for the endonucleolytic cleavage of the 5'-termini of tRNAs. Ribonuclease MRP (RNase MRP) is a ribonucleoprotein that has the ability to cleave both mitochondrial RNA primers presumed to be involved in mitochondrial DNA replication and rRNA precursors for the production of mature rRNAs. Several lines of evidence suggest that these two ribonucleoproteins are related to each other, both functionally and evolutionarily. Both of these enzymes have activity in the nucleus and mitochondria. Each cleave their RNA substrates in a divalent cation dependent manner to generate 5'-phosphate and 3'-OH termini. In addition, the RNA subunits of both complexes can be folded into a similar secondary structure. Each can be immunoprecipitated from mammalian cells with Th antibodies. In yeast, both have been found to share at least one common protein. This review will discuss some of the recent advances in our understanding of the structure, function and evolutionary relationship of these two enzymes in the yeast, Saccharomyces cerevisiae.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Conserved Sequence DNA Replication DNA, Fungal/biosynthesis DNA, Mitochondrial/biosynthesis Endoribonucleases/chemistry,metabolism Evolution, Molecular Mammals Molecular Sequence Data Nucleic Acid Conformation Protein Structure, Secondary RNA, Catalytic/chemistry,metabolism Ribonuclease P Ribonucleoproteins/chemistry,metabolism Saccharomyces cerevisiae/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
DNA, Fungal DNA, Mitochondrial RNA, Catalytic Ribonucleoproteins Endoribonucleases mitochondrial RNA-processing endoribonuclease Ribonuclease P
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reilly T H
Department of Biochemistry and Molecular Biology, SUNY Health Science Center at Syracuse, NY 13210, USA.
Schmitt M E
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00988711
Published
1995-00-00
Pages
87-93
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00988711 View journal details
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