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PMID: 8879200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Increased interleukin 4 and immunoglobulin E production in transgenic mice overexpressing NK1 T cells.

The Journal of experimental medicine ·Vol. 184 ·No. 4 ·1996-10-01 ·Pages 1285-93

Bendelac A, Hunziker RD, Lantz O

Abstract

Natural Killer (NK)1.1+ (NK1) T cells are a specialized subset of alpha/beta T cells that coexpress surface receptors that are normally associated with the NK cell lineage of the innate immune system. On recognition of the conserved, major histocompatibility complex class I-like CD1 molecule, these cells are able to release explosive bursts of interleukin 4 (IL-4), a cytokine that promotes the T helper type 2 (Th2) effector class of an immune response. A unique feature of their T cell receptor (TCR) repertoire is the expression of an invariant TCR alpha chain, V alpha 14-J alpha 281, and of a restricted but polyclonal set of V beta gene families, V beta 8, V beta 7, and V beta 2. Here, we show that transgenic expression of this TCR alpha chain during thymic development is sufficient information to bias the differentiation of mainstream thymocytes towards the NK1 developmental pathway. It markedly increases the frequency of cells with the NK1 pattern of T cell differentiation and also has drastic consequences for the selection of the V beta repertoire. Transgenic CD4 cells exhibited a 10-100-fold increase in IL-4 production on mitogen stimulation in vitro and in vivo, and baseline levels of the Th2-controlled serum immunoglobulin isotypes, IgE and IgG1, were also selectively elevated in vivo.

MeSH Terms
Animals CD4-Positive T-Lymphocytes/immunology,metabolism CD8-Positive T-Lymphocytes/immunology,metabolism Cell Differentiation Flow Cytometry Immunoglobulin E/biosynthesis Interleukin-4/biosynthesis Killer Cells, Natural/metabolism Lymphocyte Activation Mice Mice, Mutant Strains Mice, Transgenic Receptors, Antigen, T-Cell, alpha-beta/genetics T-Lymphocyte Subsets/metabolism Th2 Cells Thymus Gland/cytology,immunology
Chemicals
Receptors, Antigen, T-Cell, alpha-beta Interleukin-4 Immunoglobulin E
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bendelac A
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
Hunziker R D
Lantz O
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39 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.4.1285
Published
1996-10-01
Pages
1285-93
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192838
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.184.4.1285 PMC Full Text View journal details
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