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PMID: 8876241 Published · ppublish English Journal Article

Differential synaptic localization of two major gamma-aminobutyric acid type A receptor alpha subunits on hippocampal pyramidal cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 21 ·1996-10-15 ·Pages 11939-44

Nusser Z, Sieghart W, Benke D, Fritschy JM, Somogyi P

Abstract

Hippocampal pyramidal cells, receiving domain specific GABAergic inputs, express up to 10 different subunits of the gamma-aminobutyric acid type A (GABAA) receptor, but only 3 different subunits are needed to form a functional pentameric channel. We have tested the hypothesis that some subunits are selectively located at subsets of GABAergic synapses. The alpha 1 subunit has been found in most GABAergic synapses on all postsynaptic domains of pyramidal cells. In contrast, the alpha 2 subunit was located only in a subset of synapses on the somata and dendrites, but in most synapses on axon initial segments innervated by axo-axonic cells. The results demonstrate that molecular specialization in the composition of postsynaptic GABAA receptor subunits parallels GABAergic cell specialization in targeting synapses to a specific domain of postsynaptic cortical neurons.

MeSH Terms
Animals Axons/metabolism,ultrastructure Dendrites/metabolism,ultrastructure Hippocampus/metabolism Immune Sera Macromolecular Substances Microscopy, Immunoelectron Pyramidal Cells/metabolism,ultrastructure Rats Rats, Wistar Receptors, GABA-A/analysis,metabolism Synapses/metabolism,ultrastructure
Chemicals
Immune Sera Macromolecular Substances Receptors, GABA-A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nusser Z
Anatomical Neuropharmacology Unit, University of Oxford, United Kingdom.
Sieghart W
Benke D
Fritschy J M
Somogyi P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.21.11939
Published
1996-10-15
Pages
11939-44
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38162
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.21.11939 PMC Full Text View journal details
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