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PMID: 8840529 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human placental alkaline phosphatase as a histochemical marker of gene expression in transgenic mice.

Transgenic research ·Vol. 5 ·No. 6 ·1996-11-00 ·Pages 459-66

DePrimo SE, Stambrook PJ, Stringer JR

Abstract

The human placental alkaline phosphatase (PLAP) gene was analysed for its utility as a histochemically detectable reporter gene in transgenic mice. A reporter gene was made by linking the PLAP structural gene to an enhancer-promoter element from the human beta-actin gene. This gene was inserted into the mouse genome by transfection of embryonic stem cells, and by microinjection of fertilized eggs. Histochemical staining showed that the transgene was uniformly expressed in four of four stable ES cell lines, and in all ten tissues examined from adult animals from five lines of transgenic mice. Non-transgenic cells did not stain. These results suggest that the human PLAP gene will be of utility in studies requiring phenotypic marking of cells in tissues of mice.

MeSH Terms
Actins/genetics Alkaline Phosphatase/genetics Animals Biomarkers Cell Differentiation/genetics Embryo, Mammalian Female Gene Expression Regulation, Developmental Genetic Vectors/genetics Humans Male Mice Mice, Transgenic Microinjections Placenta/enzymology Stem Cells Tissue Distribution Transfection Transgenes
Chemicals
Actins Biomarkers Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DePrimo S E
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267, USA.
Stambrook P J
Stringer J R
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Article Info
Journal
Transgenic research
Abbr.
Transgenic Res
ISSN
0962-8819
DOI
10.1007/BF01980211
Published
1996-11-00
Pages
459-66
Language
English
Region
Netherlands
NLM ID
9209120
Subset
IM
Grants
NIEHS NIH HHS · P01-ES05652 · United States
NIEHS NIH HHS · P30 ES 06096 · United States
External Links
PubMed source DOI: 10.1007/BF01980211 View journal details
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