Home LiteratureArticle Details
PMID: 8836179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 70 kDa subunit of replication protein A is required for the G1/S and intra-S DNA damage checkpoints in budding yeast.

Nucleic acids research ·Vol. 24 ·No. 18 ·1996-09-15 ·Pages 3533-7

Longhese MP, Neecke H, Paciotti V, Lucchini G, Plevani P

Abstract

The rfa1-M2 and rfa1-M4 Saccharomyces cerevisiae mutants, which are altered in the 70 kDa subunit of replication protein A (RPA) and sensitive to UV and methyl methane sulfonate (MMS), have been analyzed for possible checkpoint defects. The G1/S and intra-S DNA damage checkpoints are defective in the rfa1-M2 mutant, since rfa1-M2 cells fail to properly delay cell cycle progression in response to UV irradiation in G1 and MMS treatment during S phase. Conversely, the G2/M DNA damage checkpoint and the S/M checkpoint are proficient in rfa1-M2 cells and all the checkpoints tested are functional in the rfa1-M4 mutant. Preventing S phase entry by alpha-factor treatment after UV irradiation in G1 does not change rfa1-M4 cell lethality, while it allows partial recovery of rfa1-M2 cell viability. Therefore, the hypersensitivity to UV and MMS treatments observed in the rfa1-M4 mutant might only be due to impairment of RPA function in DNA repair, while the rfa1-M2 mutation seems to affect both the DNA repair and checkpoint functions of Rpa70.

MeSH Terms
Cells, Cultured DNA Damage DNA Replication DNA-Binding Proteins/chemistry,physiology G1 Phase Methyl Methanesulfonate/pharmacology Molecular Weight Mutagenesis, Site-Directed Mutagens/pharmacology Nocodazole/metabolism Replication Protein A S Phase Saccharomyces cerevisiae/drug effects,genetics,radiation effects Structure-Activity Relationship Ultraviolet Rays
Chemicals
DNA-Binding Proteins Mutagens Replication Protein A Methyl Methanesulfonate Nocodazole
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Longhese M P
Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
Neecke H
Paciotti V
Lucchini G
Plevani P
References (45)
45 references, click to expand
  1. Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression.
    J Mol Biol. 1995 Dec 8;254(4):595-607 PMID: 7500336
  2. The cellular responses to DNA damage.
    Trends Cell Biol. 1995 Jan;5(1):32-40 PMID: 14731431
  3. Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1996 May 1;24(9):1669-75 PMID: 8649984
  4. Distinct roles of cdk2 and cdc2 in RP-A phosphorylation during the cell cycle.
    J Cell Sci. 1993 Nov;106 ( Pt 3):983-94 PMID: 8308077
  5. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication.
    J Biol Chem. 1990 May 5;265(13):7693-700 PMID: 2159011
  6. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  7. RPA involvement in the damage-recognition and incision steps of nucleotide excision repair.
    Nature. 1995 Apr 6;374(6522):566-9 PMID: 7700386
  8. DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast.
    J Cell Sci. 1995 Sep;108 ( Pt 9):3109-18 PMID: 8537450
  9. DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint.
    Cell. 1995 Jan 13;80(1):29-39 PMID: 7813016
  10. The DNA-activated protein kinase is required for the phosphorylation of replication protein A during simian virus 40 DNA replication.
    Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12520-4 PMID: 7809070
  11. The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast.
    Genes Dev. 1994 Oct 15;8(20):2401-15 PMID: 7958905
  12. Reconstitution of functional human single-stranded DNA-binding protein from individual subunits expressed by recombinant baculoviruses.
    Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):579-83 PMID: 8290566
  13. Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.
    Genes Dev. 1990 Jun;4(6):968-77 PMID: 2200738
  14. Cell cycle control and cancer.
    Science. 1994 Dec 16;266(5192):1821-8 PMID: 7997877
  15. Saccharomyces cerevisiae BUF protein binds to sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation.
    Mol Cell Biol. 1993 Sep;13(9):5749-61 PMID: 8355713
  16. A mutation in the gene encoding the Saccharomyces cerevisiae single-stranded DNA-binding protein Rfa1 stimulates a RAD52-independent pathway for direct-repeat recombination.
    Mol Cell Biol. 1995 Mar;15(3):1632-41 PMID: 7862154
  17. Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair.
    Genes Dev. 1994 Mar 15;8(6):652-65 PMID: 7926756
  18. Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint.
    Mol Cell Biol. 1995 Nov;15(11):6128-38 PMID: 7565765
  19. Characterization of G1 checkpoint control in the yeast Saccharomyces cerevisiae following exposure to DNA-damaging agents.
    Genetics. 1994 Oct;138(2):271-81 PMID: 7828811
  20. Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase.
    Mol Cell Biol. 1996 Jul;16(7):3235-44 PMID: 8668138
  21. Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint.
    Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11791-5 PMID: 8524850
  22. Replication of simian virus 40 origin-containing DNA in vitro with purified proteins.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1834-8 PMID: 3031654
  23. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest.
    Science. 1995 Dec 1;270(5241):1488-91 PMID: 7491494
  24. The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication.
    Mol Cell Biol. 1994 Feb;14(2):923-33 PMID: 8289832
  25. A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52.
    Mol Cell Biol. 1995 Mar;15(3):1620-31 PMID: 7862153
  26. A checkpoint regulates the rate of progression through S phase in S. cerevisiae in response to DNA damage.
    Cell. 1995 Sep 8;82(5):841-7 PMID: 7671311
  27. Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.
    J Biol Chem. 1991 Jun 25;266(18):12090-8 PMID: 2050703
  28. Replication factor A is required in vivo for DNA replication, repair, and recombination.
    Mol Cell Biol. 1994 Dec;14(12):7884-90 PMID: 7969128
  29. Recombinant replication protein A: expression, complex formation, and functional characterization.
    J Biol Chem. 1994 Apr 15;269(15):11121-32 PMID: 8157639
  30. Cellular factors required for multiple stages of SV40 DNA replication in vitro.
    EMBO J. 1988 Apr;7(4):1211-8 PMID: 2841119
  31. Checkpoints: controls that ensure the order of cell cycle events.
    Science. 1989 Nov 3;246(4930):629-34 PMID: 2683079
  32. The ionizing radiation-induced replication protein A phosphorylation response differs between ataxia telangiectasia and normal human cells.
    Mol Cell Biol. 1993 Dec;13(12):7222-31 PMID: 8246944
  33. Mammalian DNA nucleotide excision repair reconstituted with purified protein components.
    Cell. 1995 Mar 24;80(6):859-68 PMID: 7697716
  34. cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication.
    EMBO J. 1992 Jun;11(6):2189-99 PMID: 1318195
  35. Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2523-7 PMID: 2833742
  36. Phosphorylation of the p34 subunit of human single-stranded-DNA-binding protein in cyclin A-activated G1 extracts is catalyzed by cdk-cyclin A complex and DNA-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8343-7 PMID: 8078885
  37. DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe.
    Mol Gen Genet. 1995 Mar 10;246(5):561-9 PMID: 7700230
  38. Requirement for the replication protein SSB in human DNA excision repair.
    Nature. 1991 Feb 7;349(6309):538-41 PMID: 1992355
  39. Reconstitution of yeast nucleotide excision repair with purified Rad proteins, replication protein A, and transcription factor TFIIH.
    J Biol Chem. 1995 Jun 2;270(22):12973-6 PMID: 7768886
  40. Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication.
    Nature. 1989 Nov 2;342(6245):92-5 PMID: 2554144
  41. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A.
    J Biol Chem. 1993 Mar 25;268(9):6131-8 PMID: 8454588
  42. UV light-induced DNA synthesis arrest in HeLa cells is associated with changes in phosphorylation of human single-stranded DNA-binding protein.
    EMBO J. 1994 May 1;13(9):2114-23 PMID: 8187764
  43. Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.
    Proc Natl Acad Sci U S A. 1995 May 23;92(11):4907-11 PMID: 7761422
  44. From DNA damage to cell cycle arrest and suicide: a budding yeast perspective.
    Curr Opin Genet Dev. 1996 Feb;6(1):4-11 PMID: 8791492
  45. The recognition of DNA damage.
    Curr Opin Genet Dev. 1996 Feb;6(1):19-25 PMID: 8791484
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.18.3533
Published
1996-09-15
Pages
3533-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146145
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.18.3533 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2