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PMID: 8834885 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular epidemiology of fluoroquinolone-resistant Escherichia coli bloodstream isolates from patients admitted to European cancer centers.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 2 ·1996-02-00 ·Pages 387-92

Oethinger M, Conrad S, Kaifel K, Cometta A, Bille J, Klotz G, Glauser MP, Marre R, Kern WV

Abstract

Previous reports have suggested an increasing incidence of highly fluoroquinolone-resistant Escherichia coli causing bacteremia among cancer patients on prophylactic therapy. We used genotyping by pulsed-field gel electrophoresis of chromosomal DNA digests and random amplified polymorphic DNA fingerprinting to study clonal relationships among such isolates obtained at 10 cancer centers located across Europe and the Middle East. Analysis by both methods indicated that isolates from different centers were genotypically unrelated to each other. There were five centers from which more than one individual patient isolate was available, and most demonstrated significant within-center genetic diversity of strains. Strains shared among patients could be identified at two centers. At the center with the largest number of bloodstream isolates from cancer patients available, fluoroquinolone-resistant control isolates from surgical patients and fluoroquinolone-susceptible control isolates from patients admitted to medical services during the same time period were unrelated to resistant cancer patient isolates and to each other as well. A substantial number of fluoroquinolone-resistant isolates (19 of 58) were nontypeable by pulsed-field gel electrophoresis. Fluoroquinolone resistance was commonly associated with multiple antibiotic resistance to chemically unrelated antibacterial agents irrespective of the origin of the isolates.

MeSH Terms
Anti-Infective Agents/pharmacology Bacteremia/microbiology Cancer Care Facilities DNA, Bacterial/genetics Drug Resistance, Microbial Electrophoresis, Gel, Pulsed-Field Escherichia coli/drug effects,genetics Europe/epidemiology Fluoroquinolones Humans Microbial Sensitivity Tests Molecular Epidemiology Random Amplified Polymorphic DNA Technique
Chemicals
Anti-Infective Agents DNA, Bacterial Fluoroquinolones
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Oethinger M
Department of Medical Microbiology, University Hospital and Medical Center, Ulm, Germany.
Conrad S
Kaifel K
Cometta A
Bille J
Klotz G
Glauser M P
Marre R
Kern W V
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.2.387
Published
1996-02-00
Pages
387-92
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163121
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.40.2.387 PMC Full Text View journal details
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