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PMID: 8824167 Published · ppublish English Journal Article

Cytotoxic effects of anthrax lethal toxin on macrophage-like cell line J774A.1.

Current microbiology ·Vol. 33 ·No. 4 ·1996-10-00 ·Pages 224-7

Lin CG, Kao YT, Liu WT, Huang HH, Chen KC, Wang TM, Lin HC

Abstract

The cytotoxic effects of anthrax lethal toxin purified from an avirulent strain were examined on mouse macrophage-like J774A.1 cells. Cell death induced by high concentration of purified lethal toxin had the characteristics of necrosis. At lower concentrations, the toxin caused no morphological change and most of the cells were viable. Interestingly, apoptotic cells were observed when the cells were preincubated with a serine/threonine phosphatase inhibitor, calyculin A, and then exposed to a toxin concentration of 0.1 microg/ml. This is the first report that lethal toxin of the anthrax bacillus can induce both necrosis and apoptosis and that protein phosphatases are implicated in the regulation of bacterial toxin-induced apoptosis.

MeSH Terms
Animals Apoptosis Bacillus anthracis/chemistry Bacterial Toxins/pharmacology Macrophages/drug effects Marine Toxins Mice Necrosis Oxazoles/pharmacology Phosphoprotein Phosphatases/metabolism
Chemicals
Bacterial Toxins Marine Toxins Oxazoles calyculin A Phosphoprotein Phosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lin C G
Institute of Preventive Medicine, National Defense Medical Center, P. O. Box 90048-700, Taipei, Taiwan, ROC.
Kao Y T
Liu W T
Huang H H
Chen K C
Wang T M
Lin H C
References (23)
23 references, click to expand
  1. Characterization of macrophage sensitivity and resistance to anthrax lethal toxin.
    Infect Immun. 1993 Jan;61(1):245-52 PMID: 8380282
  2. Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin.
    Cancer Res. 1990 Jun 15;50(12 ):3521-5 PMID: 2160320
  3. Calcium is required for the expression of anthrax lethal toxin activity in the macrophagelike cell line J774A.1.
    Infect Immun. 1989 Jul;57(7):2107-14 PMID: 2499545
  4. Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process.
    J Biol Chem. 1986 Jun 5;261(16):7123-6 PMID: 3711080
  5. Protease activation during apoptosis: death by a thousand cuts?
    Cell. 1995 Aug 11;82(3):349-52 PMID: 7634323
  6. Novel path to apoptosis: small transmembrane pores created by staphylococcal alpha-toxin in T lymphocytes evoke internucleosomal DNA degradation.
    Infect Immun. 1994 Apr;62(4):1304-12 PMID: 8132337
  7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  8. Purification of factor I and recognition of a third factor of the anthrax toxin.
    J Gen Microbiol. 1961 Sep;26:49-63 PMID: 13916257
  9. Assays of cell viability: discrimination of cells dying by apoptosis.
    Methods Cell Biol. 1994;41:15-38 PMID: 7861963
  10. Biochemical and physiological changes induced by anthrax lethal toxin in J774 macrophage-like cells.
    Mol Biol Cell. 1992 Nov;3(11):1269-77 PMID: 1457831
  11. Calyculin A and okadaic acid: inhibitors of protein phosphatase activity.
    Biochem Biophys Res Commun. 1989 Mar 31;159(3):871-7 PMID: 2539153
  12. Lethal effects of Actinobacillus actinomycetemcomitans leukotoxin on human T lymphocytes.
    Infect Immun. 1991 Sep;59(9):3267-72 PMID: 1879940
  13. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  14. Cytocidal effects of Escherichia coli hemolysin on human T lymphocytes.
    Infect Immun. 1993 May;61(5):1715-21 PMID: 8478059
  15. Elaboration of protective antigen of Bacillus anthracis under anaerobic conditions.
    Nature. 1957 May 4;179(4566):916-7 PMID: 13430734
  16. Anthrax protective antigen interacts with a specific receptor on the surface of CHO-K1 cells.
    Infect Immun. 1991 Oct;59(10):3381-6 PMID: 1909998
  17. Apoptosis induced by bacterial pathogens.
    Microb Pathog. 1994 Oct;17(4):203-12 PMID: 7715420
  18. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
    J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PMID: 6606682
  19. Production and purification of anthrax toxin.
    Methods Enzymol. 1988;165:103-16 PMID: 3148094
  20. Inactivation of Bcl-2 by phosphorylation.
    Proc Natl Acad Sci U S A. 1995 May 9;92(10):4507-11 PMID: 7753834
  21. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  22. Protein synthesis is required for expression of anthrax lethal toxin cytotoxicity.
    Infect Immun. 1994 Jul;62(7):2958-62 PMID: 8005682
  23. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis.
    J Bacteriol. 1962 Jun;83:1274-80 PMID: 13866126
Article Info
Journal
Current microbiology
Abbr.
Curr Microbiol
ISSN
0343-8651
DOI
10.1007/s002849900104
Published
1996-10-00
Pages
224-7
Language
English
Region
United States
NLM ID
7808448
External Links
PubMed source DOI: 10.1007/s002849900104 View journal details
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