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PMID: 8816780 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rad51 expression and localization in B cells carrying out class switch recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 19 ·1996-09-17 ·Pages 10222-7

Li MJ, Peakman MC, Golub EI, Reddy G, Ward DC, Radding CM, Maizels N

Abstract

Rad51 is a highly conserved eukaryotic homolog of the prokaryotic recombination protein RecA, which has been shown to function in both recombinational repair of DNA damage and meiotic recombination in yeast. In primary murine B cells cultured with lipopolysaccharide (LPS) to stimulate heavy chain class switch recombination, Rad51 protein levels are dramatically induced. Immunofluorescent microscopy shows that anti-Rad51 antibodies stain foci that are localized within the nuclei of switching B cells. Immunohistochemical analysis of splenic sections shows that clusters of cells that stain brightly with anti-Rad51 antibodies are evident within several days after primary immunization and that Rad51 staining in vivo is confined to B cells that are switching from expression of IgM to IgG antibodies. Following switch recombination, B cells populate splenic germinal centers, where somatic hypermutation and clonal proliferation occur. Germinal center B cells are not stained by anti-Rad51 antibodies. Rad51 expression is therefore not coincident with somatic hypermutation, nor does Rad51 expression correlate simply with cell proliferation. These data suggest that Rad51, or a highly related member of the conserved RecA family, may function in class switch recombination.

MeSH Terms
Amino Acid Sequence Animals Antibodies B-Lymphocytes/drug effects,immunology Cells, Cultured Chromosome Mapping Conserved Sequence DNA-Binding Proteins/analysis,biosynthesis,immunology Fluorescent Antibody Technique Gene Expression Genes, Immunoglobulin Humans Immunization Immunoglobulin Class Switching/drug effects Immunoglobulin Heavy Chains/biosynthesis,genetics Immunohistochemistry Kinetics Lipopolysaccharides/pharmacology Mice Mice, Inbred BALB C Rad51 Recombinase Recombinant Proteins/analysis,immunology Recombination, Genetic Spleen/immunology Time Factors
Chemicals
Antibodies DNA-Binding Proteins Immunoglobulin Heavy Chains Lipopolysaccharides Recombinant Proteins RAD51 protein, human Rad51 Recombinase Rad51 protein, mouse
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Li M J
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
Peakman M C
Golub E I
Reddy G
Ward D C
Radding C M
Maizels N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.19.10222
Published
1996-09-17
Pages
10222-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38365
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039799 · United States
NHGRI NIH HHS · 5RO1HG00272 · United States
NCI NIH HHS · CA39238 · United States
NIGMS NIH HHS · GM39799 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.19.10222 PMC Full Text View journal details
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