The yeast two-hybrid system: forward and reverse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 19 ·1996-09-17 ·Pages 10001-3

White MA

Abstract

暂无摘要

MeSH Terms

Animals Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors Escherichia coli/genetics Fungal Proteins/biosynthesis,metabolism,physiology Genes, Fungal Models, Genetic Mutagenesis Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis Retinoblastoma-Binding Protein 1 Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Transcription Factors/metabolism Tumor Suppressor Protein p53/biosynthesis,metabolism

Chemicals

Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors Fungal Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Retinoblastoma-Binding Protein 1 Saccharomyces cerevisiae Proteins Transcription Factors Tumor Suppressor Protein p53

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

White M A

Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235-9039, USA. white08@utsw.swmed.edu

References (12)

12 references, click to expand

A protein linkage map of Escherichia coli bacteriophage T7.
Nat Genet. 1996 Jan;12(1):72-7 PMID: 8528255
Elimination of false positives that arise in using the two-hybrid system.
Biotechniques. 1993 Jun;14(6):920-4 PMID: 8333960
Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.
Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10315-20 PMID: 8816797
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
A novel genetic system to detect protein-protein interactions.
Nature. 1989 Jul 20;340(6230):245-6 PMID: 2547163
The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9578-82 PMID: 1946372
Genetic characterization of a mammalian protein-protein interaction domain by using a yeast reverse two-hybrid system.
Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10321-6 PMID: 8816798
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
Cell. 1993 Jul 16;74(1):205-14 PMID: 8334704
Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system.
FASEB J. 1993 Jul;7(10):957-63 PMID: 8344494
Multiple Ras functions can contribute to mammalian cell transformation.
Cell. 1995 Feb 24;80(4):533-41 PMID: 7867061
Disruption of RB/E2F-1 interaction by single point mutations in E2F-1 enhances S-phase entry and apoptosis.
Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):679-84 PMID: 8570615
Stimulation of membrane ruffling and MAP kinase activation by distinct effectors of RAS.
Science. 1996 Feb 9;271(5250):810-2 PMID: 8628998

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.19.10001

white08@utsw.swmed.edu

Published

1996-09-17

Pages

10001-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC38324

Subset

Corrections

CommentOn

PMID: 8816797

CommentOn

PMID: 8816798

External Links

PubMed source DOI: 10.1073/pnas.93.19.10001 PMC Full Text View journal details

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The yeast two-hybrid system: forward and reverse.

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