Home LiteratureArticle Details
PMID: 8816490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of Drosophila mus308, a gene involved in DNA cross-link repair with homology to prokaryotic DNA polymerase I genes.

Molecular and cellular biology ·Vol. 16 ·No. 10 ·1996-10-00 ·Pages 5764-71

Harris PV, Mazina OM, Leonhardt EA, Case RB, Boyd JB, Burtis KC

Abstract

Mutations in the Drosophila mus308 gene confer specific hypersensitivity to DNA-cross-linking agents as a consequence of defects in DNA repair. The mus308 gene is shown here to encode a 229-kDa protein in which the amino-terminal domain contains the seven conserved motifs characteristic of DNA and RNA helicases and the carboxy-terminal domain shares over 55% sequence similarity with the polymerase domains of prokaryotic DNA polymerase I-like enzymes. This is the first reported member of this family of DNA polymerases in a eukaryotic organism, as well as the first example of a single polypeptide with homology to both DNA polymerase and helicase motifs. Identification of a closely related gene in the genome of Caenorhabditis elegans suggests that this novel polypeptide may play an evolutionarily conserved role in the repair of DNA damage in eukaryotic organisms.

MeSH Terms
Amino Acid Sequence Animals Bacillus/enzymology Caenorhabditis elegans/enzymology,genetics Cloning, Molecular DNA Helicases/chemistry DNA Polymerase I/biosynthesis,chemistry,genetics DNA Repair DNA Repair Enzymes DNA-Directed DNA Polymerase Drosophila Proteins Drosophila melanogaster/enzymology,genetics Escherichia coli/enzymology Genes, Insect Models, Structural Molecular Sequence Data Protein Structure, Secondary Recombinant Proteins/biosynthesis,chemistry Saccharomyces cerevisiae/enzymology Sequence Homology, Amino Acid Streptococcus pneumoniae/enzymology
Chemicals
Drosophila Proteins Recombinant Proteins DNA Polymerase I DNA-Directed DNA Polymerase DNApol-theta protein, Drosophila DNA Helicases DNA Repair Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Harris P V
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Mazina O M
Leonhardt E A
Case R B
Boyd J B
Burtis K C
References (49)
49 references, click to expand
  1. Conserved sites in the 5'-3' exonuclease domain of Escherichia coli DNA polymerase.
    Nucleic Acids Res. 1993 Sep 11;21(18):4406-7 PMID: 8415010
  2. Evidence for at least four Fanconi anaemia genes including FACC on chromosome 9.
    Nat Genet. 1992 Jun;1(3):196-8 PMID: 1303234
  3. Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.
    Genes Dev. 1994 Jun 1;8(11):1344-55 PMID: 7926735
  4. Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I.
    J Biol Chem. 1994 Nov 18;269(46):28535-8 PMID: 7961795
  5. Function and structure relationships in DNA polymerases.
    Annu Rev Biochem. 1994;63:777-822 PMID: 7526780
  6. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  7. Deoxynucleoside triphosphate and pyrophosphate binding sites in the catalytically competent ternary complex for the polymerase reaction catalyzed by DNA polymerase I (Klenow fragment).
    J Biol Chem. 1995 Jan 27;270(4):1945-54 PMID: 7829532
  8. The hypermutability conferred by the mus308 mutation of Drosophila is not specific for cross-linking agents.
    Mutat Res. 1995 May;336(3):243-50 PMID: 7739612
  9. A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.
    Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6339-43 PMID: 7603992
  10. Crystal structure of Thermus aquaticus DNA polymerase.
    Nature. 1995 Aug 17;376(6541):612-6 PMID: 7637814
  11. XPG protein has a structure-specific endonuclease activity.
    Mutat Res. 1995 Jul;347(2):55-60 PMID: 7651464
  12. Structure-specific nuclease activity in yeast nucleotide excision repair protein Rad2.
    J Biol Chem. 1995 Dec 15;270(50):30194-8 PMID: 8530429
  13. Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA.
    Mol Cell Biol. 1993 Jul;13(7):4331-41 PMID: 8321235
  14. Repair of cross-linked DNA in Escherichia coli.
    Basic Life Sci. 1975;5B:487-95 PMID: 1103865
  15. Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens.
    Genetics. 1976 Nov;84(3):485-506 PMID: 187527
  16. Mutagen sensitivity of Drosophila melanogaster. III. X-linked loci governing sensitivity to methyl methanesulfonate.
    Mol Gen Genet. 1976 Nov 24;149(1):73-85 PMID: 189178
  17. Ecdysone-inducible functions of larval fat bodies in Drosophila.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5570-4 PMID: 103097
  18. Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae.
    Genetics. 1980 Jun;95(2):273-88 PMID: 7009316
  19. Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system.
    Mol Gen Genet. 1981;182(2):196-205 PMID: 7026973
  20. A yeast mutant specifically sensitive to bifunctional alkylation.
    Mutat Res. 1981 Nov;91(6):457-62 PMID: 7027037
  21. Third-chromosome mutagen-sensitive mutants of Drosophila melanogaster.
    Genetics. 1981 Mar-Apr;97(3-4):607-23 PMID: 6795083
  22. The fate of 8-methoxypsoralen photoinduced crosslinks in nuclear and mitochondrial yeast DNA: comparison of wild-type and repair-deficient strains.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1722-6 PMID: 6281782
  23. Postreplication repair defects in mutants of Drosophila melanogaster.
    Mol Gen Genet. 1982;186(2):289-94 PMID: 6810065
  24. Immunological method for mapping genes on Drosophila polytene chromosomes.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4381-5 PMID: 6812046
  25. Genetic control of excision of Saccharomyces cerevisiae interstrand DNA cross-links induced by psoralen plus near-UV light.
    Mol Cell Biol. 1982 Aug;2(8):939-48 PMID: 6752694
  26. Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene.
    Nucleic Acids Res. 1984 Jul 11;12(13):5495-513 PMID: 6087289
  27. Repair of psoralen-induced cross-links and monoadducts in normal and repair-deficient human fibroblasts.
    Cancer Res. 1985 Nov;45(11 Pt 1):5399-404 PMID: 4053014
  28. Action mechanism of ABC excision nuclease on a DNA substrate containing a psoralen crosslink at a defined position.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8077-81 PMID: 3534882
  29. Isolation and characterization of second chromosome mutagen-sensitive mutations in Drosophila melanogaster.
    Mutat Res. 1987 Mar;177(1):83-93 PMID: 3102956
  30. A genetic and molecular analysis of DNA repair in Drosophila.
    J Cell Sci Suppl. 1987;6:39-60 PMID: 3308924
  31. Unique cross-link and monoadduct repair characteristics of a xeroderma pigmentosum revertant cell line.
    Mutat Res. 1987 Nov;184(3):255-63 PMID: 2823132
  32. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  33. A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination.
    FEBS Lett. 1988 Aug 1;235(1-2):16-24 PMID: 2841153
  34. Pyridoxal 5'-phosphate mediated inactivation of Escherichia coli DNA polymerase I: identification of lysine-635 as an essential residue for the processive mode of DNA synthesis.
    Biochemistry. 1988 Sep 6;27(18):6710-6 PMID: 3143402
  35. In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5'-exonuclease of DNA polymerase I.
    J Biol Chem. 1989 Apr 25;264(12):6755-65 PMID: 2708342
  36. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
    Nucleic Acids Res. 1989 Jun 26;17(12):4713-30 PMID: 2546125
  37. A conserved 3'----5' exonuclease active site in prokaryotic and eukaryotic DNA polymerases.
    Cell. 1989 Oct 6;59(1):219-28 PMID: 2790959
  38. An attempt to unify the structure of polymerases.
    Protein Eng. 1990 May;3(6):461-7 PMID: 2196557
  39. mus308 mutants of Drosophila exhibit hypersensitivity to DNA cross-linking agents and are defective in a deoxyribonuclease.
    Genetics. 1990 Aug;125(4):813-9 PMID: 2397884
  40. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  41. Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.
    EMBO J. 1991 Jan;10(1):25-33 PMID: 1989886
  42. MIP1 DNA polymerase of S. cerevisiae: structural similarity with the E. coli DNA polymerase I-type enzymes.
    Nucleic Acids Res. 1991 Feb 25;19(4):955 PMID: 2017377
  43. Assessment of protein models with three-dimensional profiles.
    Nature. 1992 Mar 5;356(6364):83-5 PMID: 1538787
  44. Cloning of cDNAs for Fanconi's anaemia by functional complementation.
    Nature. 1992 Apr 30;356(6372):763-7 PMID: 1574115
  45. Characterization of the mus308 gene in Drosophila melanogaster.
    Genetics. 1993 Jan;133(1):87-96 PMID: 8417992
  46. Compilation, alignment, and phylogenetic relationships of DNA polymerases.
    Nucleic Acids Res. 1993 Feb 25;21(4):787-802 PMID: 8451181
  47. Structure of DNA polymerase I Klenow fragment bound to duplex DNA.
    Science. 1993 Apr 16;260(5106):352-5 PMID: 8469987
  48. Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases.
    Science. 1993 May 7;260(5109):778-83 PMID: 7683443
  49. Detecting subtle sequence signals: a Gibbs sampling strategy for multiple alignment.
    Science. 1993 Oct 8;262(5131):208-14 PMID: 8211139
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.10.5764
Published
1996-10-00
Pages
5764-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231577
Subset
IM
Grants
NHLBI NIH HHS · HL-50068 · United States
Databases
GENBANK
D29641, D31241, D35423, D36993, D37217, D64204, D67135, D68935, L76559, P32639, P35207, U00066, U09877, U20861, U22156, U35242, U42580, U50184, Z42372, Z49325
External Links
PubMed source DOI: 10.1128/MCB.16.10.5764 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2