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PMID: 8811086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.

Nucleic acids research ·Vol. 24 ·No. 17 ·1996-09-01 ·Pages 3332-6

Lewis JD, Görlich D, Mattaj IW

Abstract

The function in splicing of a heterodimeric nuclear cap binding complex (yCBC) from the yeast Saccharomyces cerevisiae has been examined. Immunodepletion of splicing extracts with antibodies directed against one component of the complex, yCBP80, results in the efficient co-depletion of the second component, yCBP20, producing CBC-deficient splicing extract. This extract exhibits strongly reduced splicing efficiency and similar reductions in the assembly of both spliceosomes and of the earliest defined precursors to spliceosomes, commitment complexes. The addition of highly purified yCBC substantially restores these defects. These results, together with other data, suggest that CBCs play a highly conserved role in the recognition of pre-mRNA substrates at an early step in the splicing process.

MeSH Terms
Amino Acid Sequence Conserved Sequence Nuclear Cap-Binding Protein Complex Phosphoproteins/genetics,metabolism RNA Cap-Binding Proteins RNA Precursors/metabolism RNA Splicing RNA, Fungal/metabolism RNA-Binding Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spliceosomes/metabolism
Chemicals
CBC2 protein, S cerevisiae Nuclear Cap-Binding Protein Complex Phosphoproteins RNA Cap-Binding Proteins RNA Precursors RNA, Fungal RNA-Binding Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lewis J D
European Molecular Biology Laboratory, Heidelberg, Germany.
Görlich D
Mattaj I W
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.17.3332
Published
1996-09-01
Pages
3332-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146107
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.17.3332 PMC Full Text View journal details
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