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PMID: 8807080 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isoniazid induces expression of the antigen 85 complex in Mycobacterium tuberculosis.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 7 ·1996-07-00 ·Pages 1754-6

Garbe TR, Hibler NS, Deretic V

Abstract

Exposure to isoniazid induced the expression of several secreted proteins in Mycobacterium tuberculosis H37Rv. Two-dimensional gel electrophoresis and immunoblot analyses indicated that two of the prominent isonicotinic acid hydrazide-inducible polypeptides were members of the antigen 85 complex, recently demonstrated to have mycolyltransferase activity. We postulate the existence of an intermediate, whose production is inhibited by isonicotinic acid hydrazide, which plays a negative feedback regulatory role in the metabolism of mycolic acids are revealed by the overexpression of the antigen 85 complex. The approach described here relies on analyses of differential gene expression following exposure to inhibitors and may become a more general tool in dissecting the effects of antimicrobial agents.

MeSH Terms

Acyltransferases Antigens, Bacterial/biosynthesis Antitubercular Agents/pharmacology Bacterial Proteins/biosynthesis Blotting, Western Electrophoresis, Gel, Two-Dimensional Immunoblotting Isoniazid/pharmacology Mycobacterium tuberculosis/drug effects,metabolism

Chemicals

Antigens, Bacterial Antitubercular Agents Bacterial Proteins Acyltransferases antigen 85B, Mycobacterium tuberculosis Isoniazid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Garbe T R

Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758, USA.

Hibler N S

Deretic V

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22 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.40.7.1754

Published

1996-07-00

Pages

1754-6

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC163413

Subset

Grants

NIAID NIH HHS · AI35217 · United States

External Links

PubMed source DOI: 10.1128/AAC.40.7.1754 PMC Full Text View journal details

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Isoniazid induces expression of the antigen 85 complex in Mycobacterium tuberculosis.

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