Evaluation of three newer methods for investigating protein interactions of penicillin G.

Antimicrobial agents and chemotherapy ·Vol. 11 ·No. 6 ·1977-06-00 ·Pages 993-8

Peterson LR, Gerding DN, Zinneman HH, Moore BM

Abstract

THE INTERACTION OF PENICILLIN G WITH HUMAN SERUM PROTEINS WAS EVALUATED BY THREE DIFFERENT TECHNIQUES: rate of dialysis, cross-linked dextran exclusion, and ultracentrifugation. The rate-of-dialysis technique demonstrated that penicillin G binding to serum was immediate but incompletely reversible. Cross-linked dextran adsorbed or trapped significant amounts of penicillin G, necessitating correction factors of more than 10%. Ultracentrifugation was found to be the most reliable method for quantitative protein-binding determinations of penicillins.

MeSH Terms

Blood Proteins/metabolism Dextrans Dialysis In Vitro Techniques Penicillin G/metabolism Protein Binding Ultracentrifugation

Chemicals

Blood Proteins Dextrans Penicillin G

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Peterson L R

Gerding D N

Zinneman H H

Moore B M

References (30)

30 references, click to expand

The binding of penicillin antibiotics to a human liver protein.
Arch Biochem Biophys. 1976 May;174(1):339-43 PMID: 7205
[METHODS OF DETERMINATION AND REGULARITIES IN PROTEIN BINDING OF SULFONAMIDES AND PENICILLINS].
Antibiot Chemother. 1964;12:103-34 PMID: 14262384
Binding of antibiotics to the human intracellular erythrocyte proteins hemoglobin and carbonic anhydase.
J Infect Dis. 1976 Feb;133(2):185-93 PMID: 812928
Binding of drugs to serum albumin (first of two parts).
N Engl J Med. 1976 Feb 5;294(6):311-6 PMID: 1107839
Clinical pharmacology of the new penicillins. 1. The importance of serum protein binding in determining antimicrobial activity and concentration in serum.
Clin Pharmacol Ther. 1966 Mar-Apr;7(2):166-79 PMID: 4956690
Clinical significance of protein binding of the penicillins.
Ann N Y Acad Sci. 1967 Sep 27;145(2):282-90 PMID: 4998178
The prognostic significance of penicillin serum levels and protein binding in clinical medicine. A review of current studies.
Chemotherapy. 1965-1966;10(6):339-58 PMID: 5334231
Studies on the relationship of protein binding and gel-affinity of antibiotics including penicillins.
J Antibiot (Tokyo). 1970 Oct;23(10):481-7 PMID: 5482758
The Relation of Protein Binding to the Pharmacology and Antibacterial Activity of Penicillins X, G, Dihydro F, and K.
J Bacteriol. 1947 May;53(5):581-95 PMID: 16561312
Pharmacokinetics of the penicillins in man.
Clin Pharmacokinet. 1976;1(4):297-308 PMID: 797501
Cefamandole: in vitro and clinical pharmacokinetics.
Antimicrob Agents Chemother. 1976 Nov;10(5):814-23 PMID: 1008540
Cloxacillin and nafcillin: serum binding and its relationship to antibacterial effect in mice.
J Infect Dis. 1973 Aug;128(2):170-7 PMID: 4198719
The binding of drugs by plasma proteins.
J Pharm Sci. 1968 Jun;57(6):895-918 PMID: 4876452
The binding of antibiotics to serum proteins.
Br J Pharmacol Chemother. 1965 Dec;25(3):638-50 PMID: 4958410
Protein binding methodology: comparison of equilibrium dialysis and frontal analysis chromatography in the study of salicylate binding.
Anal Biochem. 1972 Jul;48(1):80-9 PMID: 5041409
Determination of binding properties of low molecular weight substances to proteins using ultracentrifugation.
Anal Biochem. 1970 Apr;34(2):517-28 PMID: 5443676
Binding of diffusible molecules by macromolecules: rapid measurement by rate of dialysis.
J Biol Chem. 1969 Feb 25;244(4):774-7 PMID: 5768868
[Binding of sulfonamides to protein. 6. Determination of protein binding of sulfonamides by means of ultracentrifuge and gel filtration].
Arzneimittelforschung. 1965 Dec;15(12):1433-41 PMID: 5899818
[Determination of the binding of sulfonamides to serum proteins with the ultracentrifuge].
Arzneimittelforschung. 1961 Dec;11:1133-7 PMID: 13874470
INHIBITORS OF PENICILLIN BINDING TO SERUM PROTEINS.
J Lab Clin Med. 1965 Mar;65:416-31 PMID: 14261323
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
INTERACTION BETWEEN CRYSTALLINE PENICILLIN AND HUMAN PLASMA PROTEINS.
Science. 1945 Jan 19;101(2612):67-8 PMID: 17840922
Binding of antibiotics to a soluble protein from rat liver.
J Infect Dis. 1974 May;129(5):552-8 PMID: 4362999
Rapid measurement of binding of ligands by rate of dialysis.
Methods Enzymol. 1973;27:464-71 PMID: 4773291
Membrane ultrafiltration: the diafiltration technique and its application to microsolute exchange and binding phenomena.
Anal Biochem. 1968 Oct 10;26(1):151-73 PMID: 5761168
A rapid, modified ultrafiltration method for determining serum protein binding and its application to new penicillins.
J Lab Clin Med. 1965 Nov;66(5):721-32 PMID: 5844605
Steroid-protein interaction with particular reference to testosterone binding by human serum.
J Biol Chem. 1967 Jan 25;242(2):182-9 PMID: 6066733
Measurement of protein-binding phenomena by gel filtration.
Biochim Biophys Acta. 1962 Oct 8;63:530-2 PMID: 13955687
A NEW APPROACH TO STUDYING THE PROTEIN-BINDING PROPERTIES OF PENICILLINS.
Nature. 1963 Aug 24;199:758-9 PMID: 14071182
Significance of serum protein and tissue binding of antimicrobial agents.
Annu Rev Med. 1976;27:287-300 PMID: 180874

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.11.6.993

Published

1977-06-00

Pages

993-8

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC352116

Subset

External Links

PubMed source DOI: 10.1128/AAC.11.6.993 PMC Full Text View journal details

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Evaluation of three newer methods for investigating protein interactions of penicillin G.

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