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PMID: 8794366 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Helper virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells.

Journal of virology ·Vol. 70 ·No. 10 ·1996-10-00 ·Pages 7190-7

Fraefel C, Song S, Lim F, Lang P, Yu L, Wang Y, Wild P, Geller AI

Abstract

Herpes simplex virus type 1 (HSV-1) plasmid vectors have promise for genetic intervention in the brain, but several problems caused by the helper virus have compromised their utility. To develop a helper virus-free packaging system for these vectors, the DNA cleavage/packaging signals were deleted from a set of cosmids that represents the HSV-1 genome. Following cotransfection into cells, this modified cosmid set supported replication and packaging of vector DNA. However, in the absence of the DNA cleavage/packaging signals, the HSV-1 genome was not packaged, and consequently vector stocks were free of detectable helper virus. In the absence of helper virus, the vectors efficiently infected rat neural cells in culture or in the brain with minimal cytopathic effects. beta-galactosidase-positive cells were observed for at least 1 month in vivo, and vector DNA persisted for this period. This system may facilitate studies on neuronal physiology and potential therapeutic applications.

MeSH Terms
Animals Base Sequence Brain/pathology Cells, Cultured Cytopathogenic Effect, Viral Gene Transfer Techniques Genetic Therapy Genetic Vectors Herpesvirus 1, Human/genetics Molecular Sequence Data Plasmids Rats
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Fraefel C
Division of Endocrinology, Childrens's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Song S
Lim F
Lang P
Yu L
Wang Y
Wild P
Geller A I
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.10.7190-7197.1996
Published
1996-10-00
Pages
7190-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190772
Subset
IM
Grants
NIA NIH HHS · AG 10827 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.10.7190-7197.1996 PMC Full Text View journal details
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