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PMID: 8790430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 18 ·1996-09-03 ·Pages 9910-4

Stansfeld CE, Röper J, Ludwig J, Weseloh RM, Marsh SJ, Brown DA, Pongs O

Abstract

We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of calcium-dependent kinases and phosphatases. The effect of calcium on r-eag channel activity was studied in inside-out membrane patches. Calcium inhibited r-eag channel activity with a mean IC50 of 67 nM. Activation of muscarinic receptors, generating calcium oscillations in the transfected cells, induced a synchronous inhibition of r-eag mediated outward currents. This shows that calcium can mediate r-eag current inhibition following muscarinic receptor activation. The data indicate that r-eag channels are calcium-inhibitable voltage-activated potassium channels.

MeSH Terms

Animals Calcium/metabolism Cell Line Electrophysiology Ether-A-Go-Go Potassium Channels Humans Ionomycin/pharmacology Kidney/cytology,metabolism Potassium Channels/metabolism Rats Receptors, Muscarinic/metabolism Spectrometry, Fluorescence Transfection

Chemicals

Ether-A-Go-Go Potassium Channels Potassium Channels Receptors, Muscarinic Ionomycin Calcium

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Stansfeld C E

Zentrum für Moleculare Neurobiologie, Universität Hamburg, Germany.

Röper J

Ludwig J

Weseloh R M

Marsh S J

Brown D A

Pongs O

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.18.9910

Published

1996-09-03

Pages

9910-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC38528

Subset

Grants

Wellcome Trust · United Kingdom

External Links

PubMed source DOI: 10.1073/pnas.93.18.9910 PMC Full Text View journal details

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Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line.

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