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PMID: 8788275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immortalisation of human urothelial cells.

Urological research ·Vol. 23 ·No. 6 ·1995-00-00 ·Pages 377-80

Petzoldt JL, Leigh IM, Duffy PG, Sexton C, Masters JR

Abstract

A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39 degrees C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of "normal" urothelium.

MeSH Terms

Animals Antigens, Polyomavirus Transforming Cell Line, Transformed/cytology Cell Transformation, Viral Child Culture Media Epithelial Cells Female Humans Mice Mice, Nude Temperature Transfection Ureter/cytology

Chemicals

Antigens, Polyomavirus Transforming Culture Media

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Petzoldt J L

Institute of Urology and Nephrology, University College London, UK.

Leigh I M

Duffy P G

Sexton C

Masters J R

References (11)

11 references, click to expand

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Article Info

Journal

Urological research

Abbr.

Urol Res

ISSN

0300-5623

DOI

10.1007/BF00698738

Published

1995-00-00

Pages

377-80

Language

English

Region

Germany

NLM ID

0364311

Subset

External Links

PubMed source DOI: 10.1007/BF00698738 View journal details

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Immortalisation of human urothelial cells.

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