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PMID: 8784606 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Clonal diversity of oral Eikenella corrodens within individual subjects by arbitrarily primed PCR.

Journal of clinical microbiology ·Vol. 34 ·No. 7 ·1996-07-00 ·Pages 1837-9

Chen C, Ashimoto A

Abstract

The genetic diversity of 205 Eikenella corrodens isolates recovered from dental plaque, mucosal surfaces, and saliva of 24 subjects was examined by arbitrarily primed PCR. Twenty-two subjects were colonized by multiple clones (range, two to eight; mean, 3.7). This study demonstrates the utility of arbitrarily primed PCR for clonal analysis of E. corrodens and the multiclonal colonization of E. corrodens in the oral cavity.

MeSH Terms
Adult Aggressive Periodontitis/microbiology Base Sequence Child DNA Primers/genetics Dental Plaque/microbiology Eikenella corrodens/genetics,isolation & purification,pathogenicity Genetic Variation Gram-Negative Bacterial Infections/microbiology Humans Mouth Mucosa/microbiology Periodontitis/microbiology Polymerase Chain Reaction/methods Saliva/microbiology
Chemicals
DNA Primers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen C
University of Southern California School of Dentistry, Department of Periodontology, Los Angeles 90089, USA. cochcn@hsc.usc.edu
Ashimoto A
References (7)
7 references, click to expand
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  3. Comparison of arbitrarily primed polymerase chain reaction and ribotyping for subtyping Actinobacillus actinomycetemcomitans.
    Anaerobe. 1995 Apr;1(2):97-102 PMID: 16887513
  4. Clonal analysis of Porphyromonas gingivalis by the arbitrarily primed polymerase chain reaction.
    Oral Microbiol Immunol. 1994 Apr;9(2):99-103 PMID: 8008436
  5. Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans.
    Oral Microbiol Immunol. 1993 Dec;8(6):337-43 PMID: 7908736
  6. Eikenella corrodens in the human oral cavity.
    J Periodontol. 1989 Nov;60(11):611-6 PMID: 2689625
  7. Eikenella corrodens in human oral and non-oral infections: a review.
    J Periodontol. 1992 Dec;63(12):941-53 PMID: 1474466
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1996-07-00
Pages
1837-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC229131
Subset
IM
Grants
NIDCR NIH HHS · R03 DE10902-01A1 · United States
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