Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 178 ·No. 15 ·1996-08-00 ·Pages 4734-6

Blinder D, Coschigano PW, Magasanik B

Abstract

We used cells carrying plasmids causing the overproduction of Gln3p, Ure2p, or both of these proteins to elucidate the ability of Ure2p to prevent the activation of gene expression by Gln3p in cells growing in a glutamine-containing medium. Our results indicate that Ure2p probably does not interfere with the binding of the GATA factor Gln3p to GATAAG sites but acts directly on Gln3p to block its ability to activate transcription.

MeSH Terms

Base Sequence Binding Sites/genetics DNA, Fungal/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Genes, Fungal Glutathione Peroxidase Nitrogen/metabolism Prions Repressor Proteins Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors Transcriptional Activation

Chemicals

DNA, Fungal DNA-Binding Proteins Fungal Proteins GLN3 protein, S cerevisiae Prions Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Glutathione Peroxidase URE2 protein, S cerevisiae Nitrogen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Blinder D

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Coschigano P W

Magasanik B

References (6)

6 references, click to expand

Yeast mutants pleiotropically impaired in the regulation of the two glutamate dehydrogenases.
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Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
Gene. 1979 Dec;8(1):121-33 PMID: 395029
Recognition of nitrogen-responsive upstream activation sequences of Saccharomyces cerevisiae by the product of the GLN3 gene.
J Bacteriol. 1995 Jul;177(14):4190-3 PMID: 7608102
The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.
Mol Cell Biol. 1991 Feb;11(2):822-32 PMID: 1990286
Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain.
Mol Cell Biol. 1991 Dec;11(12):6216-28 PMID: 1682800
Biochemical and physiological aspects of glutamine synthetase inactivation in Saccharomyces cerevisiae.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.178.15.4734-4736.1996

Published

1996-08-00

Pages

4734-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC178249

Subset

External Links

PubMed source DOI: 10.1128/jb.178.15.4734-4736.1996 PMC Full Text View journal details

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Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae.

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