Home LiteratureArticle Details
PMID: 8754817 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

per mRNA cycling is locked to lights-off under photoperiodic conditions that support circadian feedback loop function.

Molecular and cellular biology ·Vol. 16 ·No. 8 ·1996-08-00 ·Pages 4182-8

Qiu J, Hardin PE

Abstract

Circadian fluctuations in per mRNA and protein are central to the operation of a negative feedback loop that is necessary for setting the free-running period and for entraining the circadian oscillator to light-dark cycles. In this study, per mRNA cycling and locomotor activity rhythms were measured under different light and dark cycling regimes to determine how photoperiods affect the molecular feedback loop and circadian behavior, respectively. These experiments reveal that per mRNA peaks in abundance 4 h after lights-off in photoperiods of < or = 16 h, that, phase shifts in per mRNA cycling and behavioral rhythmicity occur rapidly after flies are transferred from one photoperiod to another, and that photoperiods longer than 20 h abolish locomotor activity rhythms and leave per mRNA at a median constitutive level. These results indicate that the per feedback loop uses lights-off as a phase reference point and suggest (along with previous findings for per01 and tim01) that per mRNA cycling is not regulated via simple negative feedback from the per protein.

MeSH Terms
Animals Behavior, Animal/physiology Circadian Rhythm Drosophila Proteins Drosophila melanogaster/physiology Feedback Gene Expression Regulation Light Motor Activity Nuclear Proteins/genetics Period Circadian Proteins Photoperiod RNA, Messenger/genetics
Chemicals
Drosophila Proteins Nuclear Proteins PER protein, Drosophila Period Circadian Proteins RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Qiu J
Department of Biology and Institute of Bioscience and Technology, Center for Advanced Invertebrate Molecular Sciences, Texas A&M University, College Station 77843-3258, USA.
Hardin P E
References (31)
31 references, click to expand
  1. A promoterless period gene mediates behavioral rhythmicity and cyclical per expression in a restricted subset of the Drosophila nervous system.
    Neuron. 1994 Mar;12(3):555-70 PMID: 8155319
  2. Temporal phosphorylation of the Drosophila period protein.
    Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2260-4 PMID: 8134384
  3. Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clock.
    Neuron. 1995 Feb;14(2):365-72 PMID: 7857645
  4. An ultrashort clock mutation at the period locus of Drosophila melanogaster that reveals some new features of the fly's circadian system.
    J Biol Rhythms. 1994 Winter;9(3-4):189-216 PMID: 7772790
  5. Suppression of PERIOD protein abundance and circadian cycling by the Drosophila clock mutation timeless.
    EMBO J. 1995 Aug 15;14(16):4044-9 PMID: 7664743
  6. The effects of period mutations and light on the activity rhythms of Drosophila melanogaster.
    J Biol Rhythms. 1995 Sep;10(3):267-80 PMID: 7488764
  7. Rhythmic expression of timeless: a basis for promoting circadian cycles in period gene autoregulation.
    Science. 1995 Nov 3;270(5237):808-10 PMID: 7481772
  8. Isolation of timeless by PER protein interaction: defective interaction between timeless protein and long-period mutant PERL.
    Science. 1995 Nov 3;270(5237):811-5 PMID: 7481773
  9. A light-entrainment mechanism for the Drosophila circadian clock.
    Nature. 1996 Mar 14;380(6570):129-35 PMID: 8600384
  10. Light-induced degradation of TIMELESS and entrainment of the Drosophila circadian clock.
    Science. 1996 Mar 22;271(5256):1736-40 PMID: 8596937
  11. Resetting the Drosophila clock by photic regulation of PER and a PER-TIM complex.
    Science. 1996 Mar 22;271(5256):1740-4 PMID: 8596938
  12. Regulation of the Drosophila protein timeless suggests a mechanism for resetting the circadian clock by light.
    Cell. 1996 Mar 8;84(5):677-85 PMID: 8625406
  13. Clock mutants of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2112-6 PMID: 5002428
  14. Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.
    J Neurogenet. 1986 Sep;3(5):249-91 PMID: 3097289
  15. The period gene of Drosophila carries species-specific behavioral instructions.
    EMBO J. 1988 Dec 1;7(12):3939-47 PMID: 3208755
  16. Reciprocal behaviour associated with altered homeostasis and photosensitivity of Drosophila clock mutants.
    J Neurogenet. 1989 Sep;6(1):1-10 PMID: 2506319
  17. A new mutation at the period locus of Drosophila melanogaster with some novel effects on circadian rhythms.
    J Neurogenet. 1989 Aug;5(4):229-56 PMID: 2509652
  18. Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels.
    Nature. 1990 Feb 8;343(6258):536-40 PMID: 2105471
  19. Antibodies to the period gene product of Drosophila reveal diverse tissue distribution and rhythmic changes in the visual system.
    Neuron. 1988 Apr;1(2):141-50 PMID: 3152288
  20. Expression of a Drosophila mRNA is under circadian clock control during pupation.
    Development. 1989 Dec;107(4):869-80 PMID: 2517256
  21. Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster.
    Genetics. 1990 Jul;125(3):557-78 PMID: 2116357
  22. Circadian fluctuations of period protein immunoreactivity in the CNS and the visual system of Drosophila.
    J Neurosci. 1990 Aug;10(8):2749-62 PMID: 2117644
  23. The strength and periodicity of D. melanogaster circadian rhythms are differentially affected by alterations in period gene expression.
    Neuron. 1991 May;6(5):753-66 PMID: 1902699
  24. Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms.
    J Neurosci. 1992 Sep;12(9):3321-49 PMID: 1382123
  25. Circadian oscillations in period gene mRNA levels are transcriptionally regulated.
    Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11711-5 PMID: 1465387
  26. Behavior in light-dark cycles of Drosophila mutants that are arrhythmic, blind, or both.
    J Biol Rhythms. 1993 Spring;8(1):67-94 PMID: 8490212
  27. Phase shifting of the circadian clock by induction of the Drosophila period protein.
    Science. 1994 Jan 14;263(5144):237-40 PMID: 8284676
  28. Negative feedback defining a circadian clock: autoregulation of the clock gene frequency.
    Science. 1994 Mar 18;263(5153):1578-84 PMID: 8128244
  29. Loss of circadian behavioral rhythms and per RNA oscillations in the Drosophila mutant timeless.
    Science. 1994 Mar 18;263(5153):1603-6 PMID: 8128246
  30. Block in nuclear localization of period protein by a second clock mutation, timeless.
    Science. 1994 Mar 18;263(5153):1606-9 PMID: 8128247
  31. Constitutive overexpression of the Drosophila period protein inhibits period mRNA cycling.
    EMBO J. 1994 Aug 1;13(15):3590-8 PMID: 8062834
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.8.4182
Published
1996-08-00
Pages
4182-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231415
Subset
IM
Grants
NINDS NIH HHS · R29 NS31214 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.8.4182 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2