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题目:: Retention of HIV-1 inside infected MOLT-4 cells in association with adhesion-induced cytoskeleton reorganization.
作者:: Choudhury(S),el-Farrash(M A),Kuroda(M J),Harada(S)
状态:: 发布时间1996-09-20 , 更新时间 2006-11-15
期刊:: AIDS
摘要:: To study the mechanism of the suppression of HIV release during cell-to-cell adhesion.,To investigate the effects of cell-to-cell adhesion on HIV release in association with cytoskeletal elements, chronically HIV-infected T cells were cocultured with different adherent cell lines, cultured on a fibronectin-coated surface, or treated with cytochalasin D. The amount of viral protein released in the culture supernatant and retained inside the cells was monitored by a p24 enzyme-linked immunosorbent assay and Western blotting. For F-actin staining, cells were stained with FITC-labelled phalloidine and examined by immunofluorescence microscopy.,Cocultivation resulted in a reduced amount of virus in the culture supernatant and induced the retention of viral protein inside the infected cells. On adhesion to cells, the F-actin of the infected cells was polarized towards the cell periphery from a diffuse cytoplasmic distribution. Similar data were obtained when the infected cells were treated with cytochalasin D or adhered to fibronectin.,Cell-to-cell adhesion induced polarization of F-actin, which might facilitate HIV retention inside infected T cells.
语言:: eng
DOI:

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