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PMID: 8722574 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing of the dnaK locus in Streptomyces coelicolor A3(2).

DNA sequence : the journal of DNA sequencing and mapping ·Vol. 6 ·No. 3 ·1996-00-00 ·Pages 179-84

Brans A, Loriaux A, Joris B, Dusart J

Abstract

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics Base Sequence Cloning, Molecular Conserved Sequence/genetics DNA Probes Escherichia coli Proteins Genes, Bacterial HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins/chemistry,genetics Heat-Shock Proteins/chemistry,genetics Molecular Sequence Data Open Reading Frames/genetics Operon/genetics Polymerase Chain Reaction Sequence Homology, Amino Acid Streptomyces/chemistry,genetics
Chemicals
Bacterial Proteins DNA Probes DnaJ protein, E coli Escherichia coli Proteins GrpE protein, Bacteria HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins dnaK protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brans A
Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
Loriaux A
Joris B
Dusart J
Article Info
Journal
DNA sequence : the journal of DNA sequencing and mapping
Abbr.
DNA Seq
ISSN
1042-5179
DOI
10.3109/10425179609010207
Published
1996-00-00
Pages
179-84
Language
English
Region
England
NLM ID
9107800
Subset
IM
Databases
GENBANK
D14715, K01298, L08110, M55224, M62819, M74569, M84964, M95576, M95799, M96847, M98865, M99413, X07863, X52175, X60265, X69050, X76642, X77458
External Links
PubMed source DOI: 10.3109/10425179609010207
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