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PMID: 8709276 Published · ppublish English Journal Article

Differential glycosylation, virion incorporation, and sensitivity to neutralizing antibodies of human immunodeficiency virus type 1 envelope produced from infected primary T-lymphocyte and macrophage cultures.

Journal of virology ·Vol. 70 ·No. 9 ·1996-09-00 ·Pages 6431-6

Willey RL, Shibata R, Freed EO, Cho MW, Martin MA

Abstract

Two primary cell targets for human immunodeficiency virus type 1 (HIV-1) infection in vivo are CD4+ T lymphocytes and monocyte-derived macrophages (MDM). HIV-1 encodes envelope glycoproteins which mediate virus entry into these cells. We have utilized infected and radiolabelled primary peripheral blood mononuclear cell (PBMC) and MDM cultures to examine the biochemical and antigenic properties of the HIV-1 envelope produced in these two cell types. The gp120 produced in MDM migrates as a broad, diffuse band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels compared with that of the more homogeneous gp120 released from PBMCs. Glycosidase analyses indicated that the diffuse appearance of the MDM gp120 is due to the presence of asparagine-linked carbohydrates containing lactosaminoglycans, a modification not observed with the gp120 produced in PBMCs. Neutralization experiments, using isogeneic PBMC and MDM-derived macrophage-tropic HIV-1 isolates, indicate that 8- to 10-fold more neutralizing antibody, directed against the viral envelope, is required to block virus produced from MDM. These results demonstrate that HIV-1 released from infected PBMC and MDM cultures differs in its biochemical and antigenic properties.

MeSH Terms
Autoradiography Cells, Cultured Electrophoresis, Polyacrylamide Gel Gene Products, env/immunology Glycoside Hydrolases Glycosylation HIV Antibodies HIV Antigens/immunology HIV Envelope Protein gp120/biosynthesis,immunology,isolation & purification HIV-1/immunology,physiology HeLa Cells Humans Immunoblotting Macrophages/virology Methionine/metabolism Neutralization Tests Sensitivity and Specificity Sulfur Radioisotopes T-Lymphocytes/virology Virion/immunology,physiology
Chemicals
Gene Products, env HIV Antibodies HIV Antigens HIV Envelope Protein gp120 Sulfur Radioisotopes Methionine Glycoside Hydrolases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Willey R L
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
Shibata R
Freed E O
Cho M W
Martin M A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.9.6431-6436.1996
Published
1996-09-00
Pages
6431-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190674
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.9.6431-6436.1996 PMC Full Text View journal details
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