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PMID: 8700852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 5 ·1996-03-05 ·Pages 1875-80

Herb A, Higuchi M, Sprengel R, Seeburg PH

Abstract

RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channel's Q/R site. We show by transcript analysis of minigenes transiently expressed in PC-12 cells that, in contrast to GluR-B pre-mRNA, where the two editing sites (Q/R and R/G) require base pairing with nearby intronic editing site complementary sequences (ECSs), editing in GluR5 and GluR6 pre-mRNAs recruits an ECS located as far as 1900 nucleotides distal to the Q/R site. The exon-intron duplex structure of the GluR5 and GluR6 pre-mRNAs appears to be a substrate of double-stranded RNA-specific adenosine deaminase. This enzyme when coexpressed in HEK 293 cells preferentially targets the adenosine of the Q/R site and of an unpaired position in the ECS which is highly edited in brain.

MeSH Terms

Adenosine/chemistry Amino Acid Sequence Animals Base Sequence Brain/physiology DNA Primers/chemistry Hydrogen Bonding Introns Mice Molecular Sequence Data Nucleic Acid Conformation PC12 Cells RNA Editing RNA Precursors RNA, Double-Stranded/genetics Rats Receptors, Kainic Acid/genetics

Chemicals

DNA Primers RNA Precursors RNA, Double-Stranded Receptors, Kainic Acid Adenosine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Herb A

Laboratory for Molecular Neuroendocrinololgy, Zentrum für Molekulare Biologie, Heidelberg, Germany.

Higuchi M

Sprengel R

Seeburg P H

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.5.1875

Published

1996-03-05

Pages

1875-80

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC39875

Subset

Databases

GENBANK

U31443, U31444

External Links

PubMed source DOI: 10.1073/pnas.93.5.1875 PMC Full Text View journal details

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Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.

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