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PMID: 8691127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mechanisms of transactivation by nuclear factor of activated T cells-1.

The Journal of experimental medicine ·Vol. 184 ·No. 1 ·1996-07-01 ·Pages 141-7

Luo C, Burgeon E, Rao A

Abstract

Nuclear factor of activated T cells-family proteins (NFAT1/NFATp, NFATc, NFAT3, and NFAT4/NFATx/NFATc3) play a key role in the transcription of cytokine genes and other genes during the immune response. We have defined the mechanisms of transactivation by NFAT1. NFAT1 possesses two transactivation domains whose sequences are not conserved in the other NFAT-family proteins, and a conserved DNA-binding domain that mediates the recruitment of cooperating nuclear transcription factors even when it is expressed in the absence of other regions of the protein. The activity of the NH2-terminal transactivation domain is modulated by an adjacent regulatory region that contains several conserved sequence motifs represented only in the NFAT family. Our results emphasize the multiple levels at which NFAT-dependent transactivation is regulated, and predict significant differences in the architecture of cooperative transcription complexes containing different NFAT-family proteins.

MeSH Terms
Binding Sites DNA-Binding Proteins/physiology Gene Expression Regulation Humans Lymphocyte Activation NFATC Transcription Factors Nuclear Proteins/physiology Recombinant Fusion Proteins Structure-Activity Relationship T-Lymphocytes/physiology Transcription Factors/physiology Transcription, Genetic Transcriptional Activation Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins NFATC Transcription Factors NFATC2 protein, human NFATC3 protein, human Nuclear Proteins Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luo C
Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Burgeon E
Rao A
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36 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.1.141
Published
1996-07-01
Pages
141-7
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192690
Subset
IM
Grants
NCI NIH HHS · CA-42471 · United States
NIGMS NIH HHS · GM-46227 · United States
NIAID NIH HHS · P01 AI-35297 · United States
External Links
PubMed source DOI: 10.1084/jem.184.1.141 PMC Full Text View journal details
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