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PMID: 8678978 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3.

Mammalian genome : official journal of the International Mammalian Genome Society ·Vol. 7 ·No. 8 ·1996-08-00 ·Pages 586-90

Hwang S, Benjamin LE, Oh B, Rothstein JL, Ackerman SL, Beddington RS, Solter D, Knowles BB

Abstract

To study the molecular function of genes expressed during preimplantation development, we isolated a novel maternal transcript SSEC (Stage Specific Embryonic cDNA)-26 from a partial subtraction library of mouse unfertilized eggs and preimplantation embryos. The SSEC-26 transcript is abundant in the unfertilized egg and also actively transcribed from the newly formed zygotic genome. On the basis of its expression in eggs and embryos, this new mouse gene is named Mem (maternal-embryonic) 3. The genomic locus of Mem3 has been mapped to Chromosome (Chr) 8 near the D8Mit78 marker and the glutaryl CoA dehydrogenase (Gcdh) locus. The deduced amino acid sequence of MEM3 resembles that of the yeast VPS (Vacuolar Protein Sorting) 35 in two separate domains. A cDNA sequence of the potential human homolog of Mem3 has been assembled with partial clones from the EST database and assigned to human Chr 16.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Blastocyst Chromosome Mapping Chromosomes, Human, Pair 16 DNA, Complementary Female Gene Expression Regulation, Developmental Gene Library Glutaryl-CoA Dehydrogenase Humans Mice Molecular Sequence Data Open Reading Frames Ovum Oxidoreductases/genetics Oxidoreductases Acting on CH-CH Group Donors Proteins/genetics Saccharomyces cerevisiae/genetics Sea Urchins/genetics Sequence Homology, Amino Acid Transcription, Genetic Vesicular Transport Proteins

Chemicals

DNA, Complementary Proteins Vesicular Transport Proteins Vps35 protein, mouse Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors Glutaryl-CoA Dehydrogenase

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Hwang S

Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.

Benjamin L E

Oh B

Rothstein J L

Ackerman S L

Beddington R S

Solter D

Knowles B B

References (15)

15 references, click to expand

SpCoel1: a sea urchin profilin gene expressed specifically in coelomocytes in response to injury.
Mol Biol Cell. 1992 Apr;3(4):403-14 PMID: 1498361
Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
Genes Dev. 1987 Dec;1(10):1201-11 PMID: 3123315
Structure and function of the mannose 6-phosphate/insulinlike growth factor II receptors.
Annu Rev Biochem. 1992;61:307-30 PMID: 1323236
Cloning, structure, and chromosome localization of the mouse glutaryl-CoA dehydrogenase gene.
Genomics. 1995 Aug 10;28(3):508-12 PMID: 7490088
The fungal vacuole: composition, function, and biogenesis.
Microbiol Rev. 1990 Sep;54(3):266-92 PMID: 2215422
Gene expression during preimplantation mouse development.
Genes Dev. 1992 Jul;6(7):1190-201 PMID: 1628826
A Macintosh program for storage and analysis of experimental genetic mapping data.
Mamm Genome. 1993;4(6):303-13 PMID: 8318734
The biogenesis of lysosomes.
Annu Rev Cell Biol. 1989;5:483-525 PMID: 2557062
Vesicle-mediated protein sorting.
Annu Rev Biochem. 1992;61:471-516 PMID: 1497318
Construction of primary and subtracted cDNA libraries from early embryos.
Methods Enzymol. 1993;225:587-610 PMID: 7694045
Hybridization of nucleic acids immobilized on solid supports.
Anal Biochem. 1984 May 1;138(2):267-84 PMID: 6204550
Maps from two interspecific backcross DNA panels available as a community genetic mapping resource.
Mamm Genome. 1994 May;5(5):253-74 PMID: 8075499
Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases.
Mol Biol Cell. 1992 Apr;3(4):415-27 PMID: 1498362
Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast.
J Cell Biol. 1995 Apr;129(2):321-34 PMID: 7721937
Assignment of human glutaryl-CoA dehydrogenase gene (GCDH) to the short arm of chromosome 19 (19p13.2) by in situ hybridization and somatic cell hybrid analysis.
Genomics. 1994 May 1;21(1):289-90 PMID: 8088809

Article Info

Journal

Mammalian genome : official journal of the International Mammalian Genome Society

Abbr.

Mamm Genome

ISSN

0938-8990

DOI

10.1007/s003359900174

Published

1996-08-00

Pages

586-90

Language

English

Region

United States

NLM ID

9100916

Subset

Grants

NCI NIH HHS · P30 CA34196 · United States

NCI NIH HHS · R01 CA37225 · United States

Databases

GENBANK

U47024

External Links

PubMed source DOI: 10.1007/s003359900174 View journal details

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Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3.

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