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PMID: 8670795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neuroserpin, an axonally secreted serine protease inhibitor.

The EMBO journal ·Vol. 15 ·No. 12 ·1996-06-17 ·Pages 2944-53

Osterwalder T, Contartese J, Stoeckli ET, Kuhn TB, Sonderegger P

Abstract

We have identified and chromatographically purified an axonally secreted glycoprotein of CNS and PNS neurons. Several peptides derived from it were microsequenced. Based on these sequences, a fragment of the corresponding cDNA was amplified and used as a probe to isolate a full length cDNA from a chicken brain cDNA library. Because the deduced amino acid sequence qualified the protein as a novel member of the serpin family of serine protease inhibitors, we called it neuroserpin. Analysis of the primary structural features further characterized neuroserpin as a heparin-independent, functional inhibitor of a trypsin-like serine protease. In situ hybridization revealed a predominantly neuronal expression during the late stages of neurogenesis and in the adult brain in regions which exhibit synaptic plasticity. Thus, neuroserpin might function as an axonally secreted regulator of the local extracellular proteolysis involved in the reorganization of the synaptic connectivity during development and synapse plasticity in the adult.

MeSH Terms
Amino Acid Sequence Animals Axons/metabolism Base Sequence Cells, Cultured Chickens Chromatography, Liquid Cloning, Molecular DNA, Complementary Electrophoresis, Polyacrylamide Gel Ganglia, Spinal/metabolism Molecular Sequence Data Motor Neurons/metabolism Neuropeptides/genetics,isolation & purification,metabolism RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid Serine Proteinase Inhibitors/genetics,isolation & purification,metabolism Serpins/genetics,isolation & purification,metabolism Spinal Cord/cytology,metabolism
Chemicals
DNA, Complementary Neuropeptides RNA, Messenger Serine Proteinase Inhibitors Serpins neuroserpin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Osterwalder T
Institute of Biochemistry, University of Zurich, Switzerland.
Contartese J
Stoeckli E T
Kuhn T B
Sonderegger P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-06-17
Pages
2944-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450235
Subset
IM
Databases
GENBANK
Z71930
External Links
PubMed source PMC Full Text View journal details
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